Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis - PubMed (original) (raw)

Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis

I G Charles et al. Proc Natl Acad Sci U S A. 1989 May.

Abstract

Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein synthesis of P.69 is under genetic control of the vir locus. Using oligonucleotide probes derived from the protein sequence of a cyanogen bromide fragment, we have cloned the gene for P.69 from B. pertussis CN2992. Analysis of the DNA sequence reveals a G + C-rich gene capable of encoding a protein of 910 amino acids with a Mr of 93,478, suggesting that P.69 is a processed form of a larger precursor. In common with some of the genes in the pertussis toxin operon, the sequence CCTGG was found 5' to the ATG initiation codon. At the 3' end, 29 bases after the TAA stop codon, the sequence GTTTTTCCT was found and may have some function in transcription termination. A full-length clone of the gene for P.69 carried by the cosmid pBPI69 was unable to direct the expression of P.69 protein in an Escherichia coli host. The generation of P.69-fusion products allowed the detection of P.69-specific protein products synthesized in E. coli.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Gen Microbiol. 1970 Oct;63(2):211-20 - PubMed
    1. J Bacteriol. 1957 Aug;74(2):133-6 - PubMed
    1. Annu Rev Genet. 1979;13:319-53 - PubMed
    1. Microbiol Rev. 1980 Dec;44(4):722-38 - PubMed
    1. Gene. 1980 Nov;11(3-4):291-8 - PubMed

MeSH terms

Substances

LinkOut - more resources