Multivalent N-acetylgalactosamine-conjugated siRNA localizes in hepatocytes and elicits robust RNAi-mediated gene silencing - PubMed (original) (raw)

. 2014 Dec 10;136(49):16958-61.

doi: 10.1021/ja505986a. Epub 2014 Dec 1.

Jennifer L S Willoughby, Amy Chan, Klaus Charisse, Md Rowshon Alam, Qianfan Wang, Menno Hoekstra, Pachamuthu Kandasamy, Alexander V Kel'in, Stuart Milstein, Nate Taneja, Jonathan O'Shea, Sarfraz Shaikh, Ligang Zhang, Ronald J van der Sluis, Michael E Jung, Akin Akinc, Renta Hutabarat, Satya Kuchimanchi, Kevin Fitzgerald, Tracy Zimmermann, Theo J C van Berkel, Martin A Maier, Kallanthottathil G Rajeev, Muthiah Manoharan

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Multivalent N-acetylgalactosamine-conjugated siRNA localizes in hepatocytes and elicits robust RNAi-mediated gene silencing

Jayaprakash K Nair et al. J Am Chem Soc. 2014.

Abstract

Conjugation of small interfering RNA (siRNA) to an asialoglycoprotein receptor ligand derived from N-acetylgalactosamine (GalNAc) facilitates targeted delivery of the siRNA to hepatocytes in vitro and in vivo. The ligands derived from GalNAc are compatible with solid-phase oligonucleotide synthesis and deprotection conditions, with synthesis yields comparable to those of standard oligonucleotides. Subcutaneous (SC) administration of siRNA-GalNAc conjugates resulted in robust RNAi-mediated gene silencing in liver. Refinement of the siRNA chemistry achieved a 5-fold improvement in efficacy over the parent design in vivo with a median effective dose (ED50) of 1 mg/kg following a single dose. This enabled the SC administration of siRNA-GalNAc conjugates at therapeutically relevant doses and, importantly, at dose volumes of ≤1 mL. Chronic weekly dosing resulted in sustained dose-dependent gene silencing for over 9 months with no adverse effects in rodents. The optimally chemically modified siRNA-GalNAc conjugates are hepatotropic and long-acting and have the potential to treat a wide range of diseases involving liver-expressed genes.

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