Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria - PubMed (original) (raw)
Review
Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria
Jana Klimentová et al. Microbiol Res. 2015 Jan.
Free article
Abstract
Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host-pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed.
Keywords: Bacterial secretion; Gram-negative bacteria; Host–pathogen interaction; Outer membrane vesicles; Vaccine development.
Copyright © 2014 Elsevier GmbH. All rights reserved.
Similar articles
- Bacterial outer membrane vesicles and the host-pathogen interaction.
Kuehn MJ, Kesty NC. Kuehn MJ, et al. Genes Dev. 2005 Nov 15;19(22):2645-55. doi: 10.1101/gad.1299905. Genes Dev. 2005. PMID: 16291643 Review. - [Advances in outer membrane vesicles of gram-negative bacteria as sub-unit vaccines - A review].
Yi J, Liu Q, Kong Q. Yi J, et al. Wei Sheng Wu Xue Bao. 2016 Jun 4;56(6):911-21. Wei Sheng Wu Xue Bao. 2016. PMID: 29727152 Review. Chinese. - Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
Pérez-Cruz C, Delgado L, López-Iglesias C, Mercade E. Pérez-Cruz C, et al. PLoS One. 2015 Jan 12;10(1):e0116896. doi: 10.1371/journal.pone.0116896. eCollection 2015. PLoS One. 2015. PMID: 25581302 Free PMC article. - Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based.
Cai W, Kesavan DK, Wan J, Abdelaziz MH, Su Z, Xu H. Cai W, et al. Diagn Pathol. 2018 Dec 11;13(1):95. doi: 10.1186/s13000-018-0768-y. Diagn Pathol. 2018. PMID: 30537996 Free PMC article. Review. - Roles of bacterial membrane vesicles.
Avila-Calderón ED, Araiza-Villanueva MG, Cancino-Diaz JC, López-Villegas EO, Sriranganathan N, Boyle SM, Contreras-Rodríguez A. Avila-Calderón ED, et al. Arch Microbiol. 2015 Jan;197(1):1-10. doi: 10.1007/s00203-014-1042-7. Epub 2014 Oct 8. Arch Microbiol. 2015. PMID: 25294190 Review.
Cited by
- Isolation and Quantification of Bacterial Membrane Vesicles for Quantitative Metabolic Studies Using Mammalian Cell Cultures.
Kretschmer M, Müller J, Henke P, Otto V, Rodriguez AA, Müsken M, Jahn D, Borrero-de Acuña JM, Neumann-Schaal M, Wegner A. Kretschmer M, et al. Cells. 2023 Nov 21;12(23):2674. doi: 10.3390/cells12232674. Cells. 2023. PMID: 38067103 Free PMC article. - Novel devices for isolation and detection of bacterial and mammalian extracellular vesicles.
Malhotra S, Amin ZM, Dobhal G, Cottam S, Nann T, Goreham RV. Malhotra S, et al. Mikrochim Acta. 2021 Mar 26;188(4):139. doi: 10.1007/s00604-021-04790-5. Mikrochim Acta. 2021. PMID: 33772384 Review. - Assessment of extracellular vesicle isolation methods from human stool supernatant.
Northrop-Albrecht EJ, Taylor WR, Huang BQ, Kisiel JB, Lucien F. Northrop-Albrecht EJ, et al. J Extracell Vesicles. 2022 Apr;11(4):e12208. doi: 10.1002/jev2.12208. J Extracell Vesicles. 2022. PMID: 35383410 Free PMC article. - Outer Membrane Vesicles Facilitate Trafficking of the Hydrophobic Signaling Molecule CAI-1 between Vibrio harveyi Cells.
Brameyer S, Plener L, Müller A, Klingl A, Wanner G, Jung K. Brameyer S, et al. J Bacteriol. 2018 Jul 10;200(15):e00740-17. doi: 10.1128/JB.00740-17. Print 2018 Aug 1. J Bacteriol. 2018. PMID: 29555694 Free PMC article. - Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages.
Jung AL, Hoffmann K, Herkt CE, Schulz C, Bertrams W, Schmeck B. Jung AL, et al. J Vis Exp. 2017 Feb 22;(120):55146. doi: 10.3791/55146. J Vis Exp. 2017. PMID: 28287548 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources