Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model - PubMed (original) (raw)
Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model
Julie A K McDonald et al. J Microbiol Methods. 2015 Jan.
Abstract
In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.
Keywords: Biofilm; Chemostat; DGGE; HITChip; Human gut microbiota; Microbial ecology.
Copyright © 2014. Published by Elsevier B.V.
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