A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase - PubMed (original) (raw)
A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase
J W Wallis et al. Cell. 1989.
Abstract
A hyper-recombination mutation was isolated that causes an increase in recombination between short repeated delta sequences surrounding the SUP4-omicron gene in S. cerevisiae. The wild-type copy of this gene was cloned by complementation of one of its pleiotropic phenotypes, slow growth. DNA sequence of the clone revealed a 656 amino acid open reading frame capable of encoding a protein homologous to the bacterial type I topoisomerase. No homology was detected with previously identified eukaryotic topoisomerases. Construction of double mutants with either of the two known yeast topoisomerase genes revealed synergistic effects on growth suggesting overlapping functions. Expression of bacterial topoisomerase I in yeast can fully complement the slow growth defect of a null mutation. We have named this locus TOP3 and suggest that it defines a novel eukaryotic topoisomerase gene.
Similar articles
- The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase.
Gangloff S, McDonald JP, Bendixen C, Arthur L, Rothstein R. Gangloff S, et al. Mol Cell Biol. 1994 Dec;14(12):8391-8. doi: 10.1128/mcb.14.12.8391-8398.1994. Mol Cell Biol. 1994. PMID: 7969174 Free PMC article. - Genome rearrangement in top3 mutants of Saccharomyces cerevisiae requires a functional RAD1 excision repair gene.
Bailis AM, Arthur L, Rothstein R. Bailis AM, et al. Mol Cell Biol. 1992 Nov;12(11):4988-93. doi: 10.1128/mcb.12.11.4988-4993.1992. Mol Cell Biol. 1992. PMID: 1328869 Free PMC article. - Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I.
Sadoff BU, Heath-Pagliuso S, Castaño IB, Zhu Y, Kieff FS, Christman MF. Sadoff BU, et al. Genetics. 1995 Oct;141(2):465-79. doi: 10.1093/genetics/141.2.465. Genetics. 1995. PMID: 8647385 Free PMC article. - A new twist to the topoisomerase I problem.
Fink GR. Fink GR. Cell. 1989 Jul 28;58(2):225-6. doi: 10.1016/0092-8674(89)90832-5. Cell. 1989. PMID: 2546675 Review. No abstract available. - Structure of eukaryotic type I DNA topoisomerase.
Hsieh T, Lee MP, Brown SD. Hsieh T, et al. Adv Pharmacol. 1994;29A:191-200. doi: 10.1016/s1054-3589(08)60546-3. Adv Pharmacol. 1994. PMID: 7826858 Review. No abstract available.
Cited by
- The Use of Thioflavin T for the Estimation and Measurement of the Plasma Membrane Electric Potential Difference in Different Yeast Strains.
Peña A, Sánchez NS, Padilla-Garfias F, Ramiro-Cortés Y, Araiza-Villanueva M, Calahorra M. Peña A, et al. J Fungi (Basel). 2023 Sep 20;9(9):948. doi: 10.3390/jof9090948. J Fungi (Basel). 2023. PMID: 37755056 Free PMC article. - Metabolic Labeling of Mitochondrial Translation Products in Whole Cells and Isolated Organelles.
Maiti P, Fontanesi F. Maiti P, et al. Methods Mol Biol. 2023;2661:193-215. doi: 10.1007/978-1-0716-3171-3_12. Methods Mol Biol. 2023. PMID: 37166639 - Ferric reductase-related proteins mediate fungal heme acquisition.
Roy U, Yaish S, Weissman Z, Pinsky M, Dey S, Horev G, Kornitzer D. Roy U, et al. Elife. 2022 Oct 6;11:e80604. doi: 10.7554/eLife.80604. Elife. 2022. PMID: 36200752 Free PMC article. - Microbial Type IA Topoisomerase C-Terminal Domain Sequence Motifs, Distribution and Combination.
Diaz B, Mederos C, Tan K, Tse-Dinh YC. Diaz B, et al. Int J Mol Sci. 2022 Aug 5;23(15):8709. doi: 10.3390/ijms23158709. Int J Mol Sci. 2022. PMID: 35955842 Free PMC article. - Concentrative Nucleoside Transporter, CNT, Results in Selective Toxicity of Toyocamycin against Candida albicans.
Ojima Y, Yokota N, Tanibata Y, Nerome S, Azuma M. Ojima Y, et al. Microbiol Spectr. 2022 Aug 31;10(4):e0113822. doi: 10.1128/spectrum.01138-22. Epub 2022 Aug 1. Microbiol Spectr. 2022. PMID: 35913167 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases