Protective Effects of Berberine on Isoproterenol-Induced Acute Myocardial Ischemia in Rats through Regulating HMGB1-TLR4 Axis - PubMed (original) (raw)

Protective Effects of Berberine on Isoproterenol-Induced Acute Myocardial Ischemia in Rats through Regulating HMGB1-TLR4 Axis

Tianzhu Zhang et al. Evid Based Complement Alternat Med. 2014.

Abstract

Berberine, an isoquinoline alkaloid originally isolated from the Chinese herb Coptis chinensis (Huanglian), has been shown to display a wide array of pharmacological activities. The present study was to investigate the effects of berberine against myocardial ischemia produced in rats by isoproterenol. 50 male Sprague-Dawley rats were randomized equally into five groups: a control group, an untreated model group, berberine (30, 60 mg/kg) treatment, or propranolol (30 mg/kg). Rats were treated for 12 days and then given isoproterenol, 85 mg/kg for 2 consecutive days by subcutaneous injection. ST-segment elevation was measured after the last administration. Serum levels of creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were measured after the rats were sacrificed. The hearts were excised for determining heart weight index, microscopic examination, high mobility group box 1 (HMGB1), toll-like receptor (TLR4), prodeath protein (Bax), antideath protein (Bcl-2), and tumor necrosis factor (TNF-α) protein were determined by western blot. Berberine decreased the ST elevation induced by acute myocardial ischemia, and decreased serum levels of CK-MB, LDH, TNF-α, and IL-6. Berberine increased total superoxide dismutase (T-SOD) activity and decreased malondialdehyde (MDA) content in myocardial tissue. Berberine can regulate HMGB1-TLR4 axis to protect myocardial ischemia.

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Figures

Figure 1

Figure 1

Effect of berberine on ST-segment elevation. C (control); M (model); P (propranolol (30 mg/kg)); D (berberine (30 mg/kg)); E (berberine (60 mg/kg)). Values are expressed as means ± SDs. Compared with control, # P < 0.05, ## P < 0.01; compared with model, ∗ P < 0.05, ∗∗ P < 0.01.

Figure 2

Figure 2

Effects of berberine on CK-MB, LDH, TNF-α, and IL-6 serum levels. C (control); M (model); P (propranolol (30 mg/kg)); D (berberine (30 mg/kg)); E (berberine (60 mg/kg)). Values are expressed as means ± SDs. Compared with control, # P < 0.05, ## P < 0.01; compared with model, ∗ P < 0.05, ∗∗ P < 0.01.

Figure 3

Figure 3

Effect of berberine on heart weight indices. C (control); M (model); P (propranolol (30 mg/kg)); D (berberine (30 mg/kg)); E (berberine (60 mg/kg)). Values are expressed as means ± SDs. Compared with control, # P < 0.05, ## P < 0.01; compared with model, ∗ P < 0.05, ∗∗ P < 0.01.

Figure 4

Figure 4

Effect of berberine on myocardial histology (×200). C (control); M (model); P (propranolol (30 mg/kg)); D (berberine (30 mg/kg)); E (berberine (60 mg/kg)).

Figure 5

Figure 5

Effect of berberine on HMGB1-TLR4 axis signaling C (control); M (model); P (propranolol (30 mg/kg)); D (berberine (30 mg/kg)); E (berberine (60 mg/kg)). Values are expressed as means ± SDs. Compared with control, # P < 0.05, ## P < 0.01; compared with model, ∗ P < 0.05, ∗∗ P < 0.01.

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