IL-4 induces LFA-1 and LFA-3 expression on Burkitt's lymphoma cell lines. Requirement of additional activation by phorbol myristate acetate for induction of homotypic cell adhesions - PubMed (original) (raw)
. 1989 Sep 1;143(5):1490-8.
Affiliations
- PMID: 2547869
IL-4 induces LFA-1 and LFA-3 expression on Burkitt's lymphoma cell lines. Requirement of additional activation by phorbol myristate acetate for induction of homotypic cell adhesions
F Rousset et al. J Immunol. 1989.
Abstract
LFA-1 and LFA-3 expression is absent or low on Burkitt's lymphoma cell lines and low on the EBV-transformed B cell line UD61. Incubation of cells of BL2 and of UD61 with various concentrations of IL-4 resulted in induction of LFA-1 and LFA-3 expression in a dose dependent fashion. This effect was already observed after 16 h of incubation whereas maximal expression was obtained after 72 h. Induction of LFA-1 and LFA-3 expression seemed to be specific for IL-4, because IL-1, IL-2, IL-3, IFN-alpha, IFN-gamma and a low m.w. B cell growth factor were ineffective. LFA-1 and LFA-3 induction by IL-4 was blocked specifically by an anti-IL-4 antiserum. Induction of LFA-1 expression by IL-4 was furthermore confirmed at the specific LFA-1 beta-chain mRNA level. IL-4 was unable to induce LFA-1 expression on EBV-transformed lymphoblastoid cell lines of two LFA-1-deficient patients. BL2 grows as single cells, but induction of LFA-1 and LFA-3 expression by IL-4 was insufficient to induce homotypic cell adhesions and required PMA as a second signal. PMA alone did not induce LFA-1 antigen expression and was unable to induce adhesions between BL2 cells in the absence of IL-4 in 22 h assays. Addition of PMA to BL2 cells that expressed LFA-1 Ag upon incubation with IL-4 resulted in aggregate formation within 30 min. Adhesions between BL2 cells induced by IL-4 in combination with PMA were blocked by anti-LFA-1 beta or anti-LFA-1 alpha-chains mAb. In addition, these mAbs dispersed preformed aggregates of BL2 cells. Our results indicate that IL-4 can induce the adhesion molecules LFA-1 and LFA-3 on B cell lines, but that an additional activation signal provided by PMA was required for the induction of homotypic cell adhesions.
Similar articles
- Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma.
Rousset F, Malefijt RW, Slierendregt B, Aubry JP, Bonnefoy JY, Defrance T, Banchereau J, de Vries JE. Rousset F, et al. J Immunol. 1988 Apr 15;140(8):2625-32. J Immunol. 1988. PMID: 2965726 - IFN-alpha induces homotypic adhesion and Leu-13 expression in human B lymphoid cells.
Evans SS, Collea RP, Leasure JA, Lee DB. Evans SS, et al. J Immunol. 1993 Feb 1;150(3):736-47. J Immunol. 1993. PMID: 8423337 - CD4 binding to major histocompatibility complex class II antigens induces LFA-1-dependent and -independent homotypic adhesion of B lymphocytes.
Kansas GS, Cambier JC, Tedder TF. Kansas GS, et al. Eur J Immunol. 1992 Jan;22(1):147-52. doi: 10.1002/eji.1830220122. Eur J Immunol. 1992. PMID: 1730248 - Hypermutation in human B cells in vivo and in vitro.
Weller S, Faili A, Aoufouchi S, Guéranger Q, Braun M, Reynaud CA, Weill JC. Weller S, et al. Ann N Y Acad Sci. 2003 Apr;987:158-65. doi: 10.1111/j.1749-6632.2003.tb06044.x. Ann N Y Acad Sci. 2003. PMID: 12727635 Review.
Cited by
- Post-transplant lymphoproliferative disease (PTLD): lymphokine production and PTLD.
Tosato G, Teruya-Feldstein J, Setsuda J, Pike SE, Jones KD, Jaffe ES. Tosato G, et al. Springer Semin Immunopathol. 1998;20(3-4):405-23. doi: 10.1007/BF00838052. Springer Semin Immunopathol. 1998. PMID: 9870254 Review. No abstract available. - Interleukin-4. A regulatory protein.
Jansen JH, Fibbe WE, Willemze R, Kluin-Nelemans JC. Jansen JH, et al. Blut. 1990 May;60(5):269-74. doi: 10.1007/BF01736226. Blut. 1990. PMID: 2190652 Review. - CD58 Immunobiology at a Glance.
Zhang Y, Liu Q, Yang S, Liao Q. Zhang Y, et al. Front Immunol. 2021 Jun 8;12:705260. doi: 10.3389/fimmu.2021.705260. eCollection 2021. Front Immunol. 2021. PMID: 34168659 Free PMC article. Review. - Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are effectors of phenotypic changes in B lymphocytes: EBNA-2 and LMP1 cooperatively induce CD23.
Wang F, Gregory C, Sample C, Rowe M, Liebowitz D, Murray R, Rickinson A, Kieff E. Wang F, et al. J Virol. 1990 May;64(5):2309-18. doi: 10.1128/JVI.64.5.2309-2318.1990. J Virol. 1990. PMID: 2157887 Free PMC article. - Rapid cytokine up-regulation of integrins, complement receptor 1 and HLA-DR on monocytes but not on lymphocytes.
Limb GA, Hamblin AS, Wolstencroft RA, Dumonde DC. Limb GA, et al. Immunology. 1992 Sep;77(1):88-94. Immunology. 1992. PMID: 1356917 Free PMC article.