Antisera specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the Na,K-ATPase: differential expression of alpha and beta subunits in rat tissue membranes - PubMed (original) (raw)
. 1989 May 30;28(11):4531-5.
doi: 10.1021/bi00437a002.
Affiliations
- PMID: 2548580
- DOI: 10.1021/bi00437a002
Antisera specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the Na,K-ATPase: differential expression of alpha and beta subunits in rat tissue membranes
A W Shyjan et al. Biochemistry. 1989.
Abstract
We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue microsomes revealed that alpha 1 subunits were expressed in all tissues while alpha 2 subunits were expressed in brain, heart, and lung. The alpha 3 subunit, a protein whose existence had been inferred from cDNA cloning, was expressed primarily in brain and copurified with ouabain-inhibitable Na,K-ATPase activity. An antiserum specific for the rat Na,K-ATPase beta subunit was generated from a TrpE-beta subunit fusion protein. Western blot analysis showed that beta subunits were present in kidney, brain, and heart. However, no beta subunits were detected in liver, lung, spleen, thymus, or lactating mammary gland. The distinct tissue distributions of alpha and beta subunits suggest that different members of the Na,K-ATPase family may have specialized functions.
Similar articles
- The Na,K-ATPase beta 2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity.
Shyjan AW, Gottardi C, Levenson R. Shyjan AW, et al. J Biol Chem. 1990 Mar 25;265(9):5166-9. J Biol Chem. 1990. PMID: 2156863 - Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA.
Schneider JW, Mercer RW, Caplan M, Emanuel JR, Sweadner KJ, Benz EJ Jr, Levenson R. Schneider JW, et al. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6357-61. doi: 10.1073/pnas.82.18.6357. Proc Natl Acad Sci U S A. 1985. PMID: 2994074 Free PMC article. - Differential expression and enzymatic properties of the Na+,K(+)-ATPase alpha 3 isoenzyme in rat pineal glands.
Shyjan AW, Ceña V, Klein DC, Levenson R. Shyjan AW, et al. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1178-82. doi: 10.1073/pnas.87.3.1178. Proc Natl Acad Sci U S A. 1990. PMID: 2153972 Free PMC article. - A new electrophoretic variant of alpha subunit of Na+/K(+)-ATPase from the submandibular gland of rats.
Kurihara K, Hosoi K, Kodama A, Ueha T. Kurihara K, et al. Biochim Biophys Acta. 1990 Jun 19;1039(2):234-40. doi: 10.1016/0167-4838(90)90191-h. Biochim Biophys Acta. 1990. PMID: 2163680 - Non-receptor protein tyrosine kinases of normal tissues.
Srivastava AK. Srivastava AK. Int J Biochem. 1990;22(11):1229-34. doi: 10.1016/0020-711x(90)90303-k. Int J Biochem. 1990. PMID: 2257948 Review. No abstract available.
Cited by
- A Transgenic Mouse Model to Selectively Identify α3 Na,K-ATPase Expressing Cells in the Nervous System.
Dobretsov M, Hayar A, Kockara NT, Kozhemyakin M, Light KE, Patyal P, Pierce DR, Wight PA. Dobretsov M, et al. Neuroscience. 2019 Feb 1;398:274-294. doi: 10.1016/j.neuroscience.2018.07.018. Epub 2018 Jul 19. Neuroscience. 2019. PMID: 30031123 Free PMC article. - The Structure and Function of the Na,K-ATPase Isoforms in Health and Disease.
Clausen MV, Hilbers F, Poulsen H. Clausen MV, et al. Front Physiol. 2017 Jun 6;8:371. doi: 10.3389/fphys.2017.00371. eCollection 2017. Front Physiol. 2017. PMID: 28634454 Free PMC article. Review. - Na+, K+-ATPase β1 subunit associates with α1 subunit modulating a "higher-NKA-in-hyposmotic media" response in gills of euryhaline milkfish, Chanos chanos.
Hu YC, Chu KF, Yang WK, Lee TH. Hu YC, et al. J Comp Physiol B. 2017 Oct;187(7):995-1007. doi: 10.1007/s00360-017-1066-9. Epub 2017 Mar 10. J Comp Physiol B. 2017. PMID: 28283795 - Age-dependent decline in density of human nerve and spinal ganglia neurons expressing the α3 isoform of Na/K-ATPase.
Romanovsky D, Mrak RE, Dobretsov M. Romanovsky D, et al. Neuroscience. 2015 Dec 3;310:342-53. doi: 10.1016/j.neuroscience.2015.09.034. Epub 2015 Sep 18. Neuroscience. 2015. PMID: 26386295 Free PMC article. - Sodium-pump gene-expression, protein abundance and enzyme activity in isolated nephron segments of the aging rat kidney.
Scherzer P, Gal-Moscovici A, Sheikh-Hamad D, Popovtzer MM. Scherzer P, et al. Physiol Rep. 2015 Jun;3(6):e12369. doi: 10.14814/phy2.12369. Physiol Rep. 2015. PMID: 26056060 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources