Insertion elements and deletion formation in a halophilic archaebacterium - PubMed (original) (raw)

Insertion elements and deletion formation in a halophilic archaebacterium

F Pfeifer et al. J Bacteriol. 1989 Sep.

Abstract

Deletion events that occur spontaneously in 36-kilobase-pair (kbp) plasmid pHH4 from the archaebacterium Halobacterium halobium were investigated. Four different deletion derivatives with sizes ranging from 5.7 to 17 kbp were isolated. Three of these deletion variants derived from pHH4 (pHH6 [17 kbp], pHH7 [16 kbp], and pHH8 [6.3 kbp]), whereas the 5.7-kbp plasmid pHH9 derived from pHH6. Strains containing pHH6, pHH7, or pHH9 each lacked the parental plasmid pHH4, while pHH8 occurred at a 1:1 ratio together with pHH4. Common to all of these plasmids was the 5.7-kbp region of pHH9 DNA. The regions containing the fusion site in the deletion derivatives were investigated and compared with the corresponding area of the parental plasmid. Each deletion occurred exactly at the terminus of an insertion element. In pHH6 and pHH7, a halobacterial insertion element (ISH2) was located at the deletion site. The DNA fused to ISH2 displayed a 7-base-pair (bp) (pHH7) or 10-bp (pHH6) sequence homology to the inverted repeat of ISH2. In the two smaller plasmids, pHH8 and pHH9, an ISH27 element was located at the deletion site. Most likely, all of these smaller plasmids resulted from an intramolecular transposition event. The ISH27 insertion sequence contains a 16-bp terminal inverted repeat and duplicates 5 bp of target DNA during the transposition with the specificity 5'ANNNT3'. Four ISH27 copies were analyzed, and two ISH27 element types were identified that have approximately 85% sequence similarity. The ISH27 insertion elements constitute a family which is related to the ISH51 family characterized for H. volcanii, another halophilic archaebacterium.

PubMed Disclaimer

Cited by

Plasmid pHH1 of Halobacterium salinarium: characterization of the replicon region, the gas vesicle gene cluster and insertion elements.
Pfeifer F, Ghahraman P. Pfeifer F, et al. Mol Gen Genet. 1993 Apr;238(1-2):193-200. doi: 10.1007/BF00279547. Mol Gen Genet. 1993. PMID: 8386798
Conservation of chromosomal arrangement among three strains of the genetically unstable archaeon Halobacterium salinarium.
Hackett NR, Bobovnikova Y, Heyrovska N. Hackett NR, et al. J Bacteriol. 1994 Dec;176(24):7711-8. doi: 10.1128/jb.176.24.7711-7718.1994. J Bacteriol. 1994. PMID: 8002597 Free PMC article.
Distribution, formation and regulation of gas vesicles.
Pfeifer F. Pfeifer F. Nat Rev Microbiol. 2012 Oct;10(10):705-15. doi: 10.1038/nrmicro2834. Epub 2012 Sep 3. Nat Rev Microbiol. 2012. PMID: 22941504 Review.
Structure of the gas vesicle plasmid in Halobacterium halobium: inversion isomers, inverted repeats, and insertion sequences.
Ng WL, Kothakota S, DasSarma S. Ng WL, et al. J Bacteriol. 1991 Mar;173(6):1958-64. doi: 10.1128/jb.173.6.1958-1964.1991. J Bacteriol. 1991. PMID: 1848217 Free PMC article.
Copy number variation is associated with gene expression change in archaea.
Dulmage KA, Darnell CL, Vreugdenhil A, Schmid AK. Dulmage KA, et al. Microb Genom. 2018 Sep;4(9):e000210. doi: 10.1099/mgen.0.000210. Epub 2018 Aug 24. Microb Genom. 2018. PMID: 30142055 Free PMC article.

References

1. Nucleic Acids Res. 1983 Nov 25;11(22):8019-30 - PubMed
1. J Bacteriol. 1988 Aug;170(8):3718-24 - PubMed
1. Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 - PubMed
1. Cell. 1982 Jan;28(1):155-63 - PubMed
1. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1017-20 - PubMed

Insertion elements and deletion formation in a halophilic archaebacterium - PubMed (original) (raw)