Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence - PubMed (original) (raw)

Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence

J C Hinton et al. Mol Gen Genet. 1989 May.

Abstract

The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.

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References

1. 1. J Bacteriol. 1985 Oct;164(1):51-6 - PubMed
2. 1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
3. 1. Gene. 1984 Feb;27(2):131-49 - PubMed
4. 1. Appl Environ Microbiol. 1985 Jul;50(1):1-9 - PubMed
5. 1. Genetics. 1979 Jul;92(3):741-7 - PubMed

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