Storage lipid synthesis is necessary for autophagy induced by nitrogen starvation - PubMed (original) (raw)

. 2015 Jan 16;589(2):269-76.

doi: 10.1016/j.febslet.2014.11.050. Epub 2014 Dec 12.

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Storage lipid synthesis is necessary for autophagy induced by nitrogen starvation

Dan Li et al. FEBS Lett. 2015.

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Abstract

Nitrogen starvation is a universal stimulus of autophagy. At present, little is known about the relationship between carbon metabolism and autophagy under nitrogen starvation. Here, we show that yeast cells continue to consume glucose and downregulate fermentation under nitrogen starvation. Storage lipid production is increased, with concurrent proliferation of lipid droplets. Furthermore, we provide evidence that triacylglycerol synthesis is crucial for autophagosome biogenesis. It is involved in a step downstream of PAS (phagophore assembly site) scaffold assembly, and upstream of the recruitment of Atg1, Atg14, Atg5 and Atg8. Finally, we demonstrate that lipid droplets transiently interact with Atg8-containing membranes. Our study reveals a novel connection linking neutral lipid metabolism, lipid droplets and autophagy.

Keywords: Autophagy; Glucose; Lipid droplet; Nitrogen starvation; Triacylglycerol; Yeast.

Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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