Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines - PubMed (original) (raw)
Comparative Study
. 1989 Sep 15;143(6):1859-67.
Affiliations
- PMID: 2550545
Comparative Study
Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines
J Browning et al. J Immunol. 1989.
Abstract
The relative ability of TNF and lymphotoxin (LT) to inhibit the growth of five human tumor cell lines was examined both in the presence and absence of IFN-gamma. Two adenocarcinoma lines, HT-29 and SK-CO-1, were 20- and 320-fold more sensitive to the inhibitory effects of TNF and LT in 3- to 4-day proliferation assays. In contrast, the breast carcinoma line BT-20 showed only a one- to twofold difference. The MCF-7 and ME-180 cell lines exhibited intermediate behavior. These results parallel the reported disparate potencies of TNF and LT in their effects on endothelial cells, hematopoietic development and their abilities to sustain a mixed lymphocyte response. Radiolabeled TNF binding studies showed two classes of receptors (Kd 0.04 to 0.15 nM and 0.2 to 1.0 nM) on the highly sensitive SK-CO-1 line. HT-29 cells also appeared to possess some high affinity-binding sites, whereas the BT-20 line completely lacked the high affinity form. Thus the presence of high affinity-binding sites correlated with increased sensitivity to the antiproliferative effects of TNF. Cold TNF competed with the binding of radiolabeled human TNF three- to fivefold better than LT for binding to all three lines. These relatively small differences between the TNF and LT receptor-binding characteristics are insufficient to explain the dramatic disparity in their antiproliferative properties. Likewise, the absolute concentrations of the unlabeled cytokines necessary to block the binding of 125I-TNF were 10- to 150-fold higher than the levels necessary to elicit the biologic response. Thus, the receptor binding data conflict with the growth inhibitory effects. This discrepancy is discussed in terms of either separate receptors for TNF and LT or more complex phenomena such as receptor cooperativity possibly resulting from multivalent interactions with the trimeric form of TNF.
Similar articles
- Differences in the biological activity of TNF alpha and TNF beta correlate with their different abilities for binding to the target cells.
Kircheis R, Milleck J, Korobko VG, Shingarova LN, Schmidt HE. Kircheis R, et al. Eur Cytokine Netw. 1992 Jul-Aug;3(4):381-90. Eur Cytokine Netw. 1992. PMID: 1330049 - Mechanisms of action of the tumor necrosis factor and lymphotoxin ligand-receptor system.
Warzocha K, Bienvenu J, Coiffier B, Salles G. Warzocha K, et al. Eur Cytokine Netw. 1995 Mar-Apr;6(2):83-96. Eur Cytokine Netw. 1995. PMID: 7578992 Review. - Tumor necrosis factor alpha: a multifaceted peptide hormone.
Rosenblum MG, Donato NJ. Rosenblum MG, et al. Crit Rev Immunol. 1989;9(1):21-44. Crit Rev Immunol. 1989. PMID: 2539946 Review.
Cited by
- Distinct roles for LTalpha3 and LTalpha1beta2 produced by B cells contribute to their multi-faceted impact on ileitis.
Randolph G, Erlich E, Czepielewski R, Field R, Dunning T, Saleh L, Hoofnagle M, Tumanov A, Guilak F, Brestoff J. Randolph G, et al. Res Sq [Preprint]. 2024 Feb 26:rs.3.rs-3962916. doi: 10.21203/rs.3.rs-3962916/v1. Res Sq. 2024. PMID: 38464070 Free PMC article. Preprint. - Does nutrition support during chemotherapy increase long-term survival of cancer patients? Lessons from the past and future perspectives.
Bozzetti F. Bozzetti F. Support Care Cancer. 2021 Dec;29(12):7269-7277. doi: 10.1007/s00520-021-06213-w. Epub 2021 Jul 26. Support Care Cancer. 2021. PMID: 34309702 Review. - Oligomeric state of the oxalate transporter, OxlT.
Kang DC, Venkataraman PA, Dumont ME, Maloney PC. Kang DC, et al. Biochemistry. 2011 Oct 4;50(39):8445-53. doi: 10.1021/bi201175y. Epub 2011 Sep 8. Biochemistry. 2011. PMID: 21866906 Free PMC article. - Reversible pH lability of cross-linked vault nanocapsules.
Yu M, Ng BC, Rome LH, Tolbert SH, Monbouquette HG. Yu M, et al. Nano Lett. 2008 Oct;8(10):3510-5. doi: 10.1021/nl080536z. Epub 2008 Sep 20. Nano Lett. 2008. PMID: 18803423 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources