Molecular cloning of SNM1, a yeast gene responsible for a specific step in the repair of cross-linked DNA - PubMed (original) (raw)
Molecular cloning of SNM1, a yeast gene responsible for a specific step in the repair of cross-linked DNA
E Haase et al. Mol Gen Genet. 1989 Jul.
Abstract
We have isolated yeast gene SNM1 via complementation of sensitivity towards bi- and tri-functional alkylating agents in haploid and diploid yeast DNA repair-deficient snm1-1 mutants. Four independent clones of plasmid DNA containing the SNM1 locus were isolated after transformation with a YEp24-based yeast gene bank. Subcloned SNM1-containing DNA showed (i) complementation of the repair-deficiency phenotype caused by either one of the two different mutant alleles snm1-1 and snm1-2ts; (ii) complementation in haploid and diploid yeast snm1-1 mutants by either single or multiple copies of the SNM1 locus; and (iii) that the SNM1 gene is at most 2.4 kb in size. Expression of SNM1 on the smallest subclone, however, was under the control of the GAL1 promotor. Gene size and direction of transcription was further verified by mutagenesis of SNM1 by Tn10-LUK transposon insertion. Five plasmids containing Tn10-LUK insertions at different sites of the SNM1-containing DNA were able to disrupt the function of genomic SNM1 after gene transplacement. Correct integration of the disrupted SNM1::Tn10-LUK at the genomic site of SNM1 was verified via tetrad analysis of the sporulated diploid obtained after mating of the SNM1::Tn10-LUK transformant to a haploid strain containing the URA3 SNM1 wild-type alleles. The size of the poly(A)+ RNA transcript of the SNM1 gene is 1.1 kb as determined by Northern analysis.
Similar articles
- Molecular structure of the DNA cross-link repair gene SNM1 (PSO2) of the yeast Saccharomyces cerevisiae.
Richter D, Niegemann E, Brendel M. Richter D, et al. Mol Gen Genet. 1992 Jan;231(2):194-200. doi: 10.1007/BF00279791. Mol Gen Genet. 1992. PMID: 1736091 - A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes.
Huisman O, Raymond W, Froehlich KU, Errada P, Kleckner N, Botstein D, Hoyt MA. Huisman O, et al. Genetics. 1987 Jun;116(2):191-9. doi: 10.1093/genetics/116.2.191. Genetics. 1987. PMID: 3038670 Free PMC article. - Vectors for cloning in yeast.
Hinnen A, Meyhack B. Hinnen A, et al. Curr Top Microbiol Immunol. 1982;96:101-17. doi: 10.1007/978-3-642-68315-2_7. Curr Top Microbiol Immunol. 1982. PMID: 6276087 Review. No abstract available. - Mutations that affect nuclear organization in yeast.
Sperry AO, Fishel BR, Garrard WT. Sperry AO, et al. Methods Cell Biol. 1991;35:525-41. doi: 10.1016/s0091-679x(08)60586-5. Methods Cell Biol. 1991. PMID: 1779866 Review. No abstract available.
Cited by
- The multifunctional SNM1 gene family: not just nucleases.
Yan Y, Akhter S, Zhang X, Legerski R. Yan Y, et al. Future Oncol. 2010 Jun;6(6):1015-29. doi: 10.2217/fon.10.47. Future Oncol. 2010. PMID: 20528238 Free PMC article. Review. - Snm1B/Apollo mediates replication fork collapse and S Phase checkpoint activation in response to DNA interstrand cross-links.
Bae JB, Mukhopadhyay SS, Liu L, Zhang N, Tan J, Akhter S, Liu X, Shen X, Li L, Legerski RJ. Bae JB, et al. Oncogene. 2008 Aug 28;27(37):5045-56. doi: 10.1038/onc.2008.139. Epub 2008 May 12. Oncogene. 2008. PMID: 18469862 Free PMC article. - hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage.
Richie CT, Peterson C, Lu T, Hittelman WN, Carpenter PB, Legerski RJ. Richie CT, et al. Mol Cell Biol. 2002 Dec;22(24):8635-47. doi: 10.1128/MCB.22.24.8635-8647.2002. Mol Cell Biol. 2002. PMID: 12446782 Free PMC article. - Assigning a function to a conserved group of proteins: the tRNA 3'-processing enzymes.
Schiffer S, Rösch S, Marchfelder A. Schiffer S, et al. EMBO J. 2002 Jun 3;21(11):2769-77. doi: 10.1093/emboj/21.11.2769. EMBO J. 2002. PMID: 12032089 Free PMC article. - Disruption of mouse SNM1 causes increased sensitivity to the DNA interstrand cross-linking agent mitomycin C.
Dronkert ML, de Wit J, Boeve M, Vasconcelos ML, van Steeg H, Tan TL, Hoeijmakers JH, Kanaar R. Dronkert ML, et al. Mol Cell Biol. 2000 Jul;20(13):4553-61. doi: 10.1128/MCB.20.13.4553-4561.2000. Mol Cell Biol. 2000. PMID: 10848582 Free PMC article.
References
- Genetics. 1981 Mar-Apr;97(3-4):607-23 - PubMed
- J Cell Sci Suppl. 1987;6:97-110 - PubMed
- Mutat Res. 1984 Jan;133(1):51-85 - PubMed
- Mutat Res. 1981 Nov;91(6):457-62 - PubMed
- Genetics. 1987 Jun;116(2):191-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials