Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerases alpha and delta - PubMed (original) (raw)

Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerases alpha and delta

M K Kenny et al. Proc Natl Acad Sci U S A. 1989 Dec.

Abstract

The human single-stranded-DNA binding protein (human SSB) is required for simian virus 40 (SV40) DNA replication in vitro. SV40 large tumor antigen and human SSB can support extensive unwinding of SV40 origin-containing DNA in the presence of ATP and a topoisomerase that relieves positive superhelicity. Although SSBs from viral and prokaryotic sources substituted for human SSB in the DNA-unwinding reaction, they did not substitute in the replication of SV40 DNA. The specificity for human SSB in SV40 DNA replication can be explained, at least in part, by the finding that DNA polymerase alpha was stimulated 10-fold by human SSB but not by other SSBs. Human SSB also stimulated proliferating-cell nuclear antigen-dependent DNA polymerase delta; however, other SSBs stimulated this polymerase as well.

PubMed Disclaimer

Similar articles

• Studies on the initiation and elongation reactions in the simian virus 40 DNA replication system.
  Matsumoto T, Eki T, Hurwitz J. Matsumoto T, et al. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9712-6. doi: 10.1073/pnas.87.24.9712. Proc Natl Acad Sci U S A. 1990. PMID: 2175912 Free PMC article.
• The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication.
  Kenny MK, Schlegel U, Furneaux H, Hurwitz J. Kenny MK, et al. J Biol Chem. 1990 May 5;265(13):7693-700. J Biol Chem. 1990. PMID: 2159011
• Synthesis of DNA containing the simian virus 40 origin of replication by the combined action of DNA polymerases alpha and delta.
  Lee SH, Eki T, Hurwitz J. Lee SH, et al. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7361-5. doi: 10.1073/pnas.86.19.7361. Proc Natl Acad Sci U S A. 1989. PMID: 2571990 Free PMC article.
• The in vitro replication of DNA containing the SV40 origin.
  Hurwitz J, Dean FB, Kwong AD, Lee SH. Hurwitz J, et al. J Biol Chem. 1990 Oct 25;265(30):18043-6. J Biol Chem. 1990. PMID: 1976634 Review. No abstract available.
• Properties of DNA polymerases delta and epsilon, and their roles in eukaryotic DNA replication.
  Bambara RA, Jessee CB. Bambara RA, et al. Biochim Biophys Acta. 1991 Jan 17;1088(1):11-24. doi: 10.1016/0167-4781(91)90147-e. Biochim Biophys Acta. 1991. PMID: 1846563 Review. No abstract available.

Cited by

• A role for the human single-stranded DNA binding protein HSSB/RPA in an early stage of nucleotide excision repair.
  Coverley D, Kenny MK, Lane DP, Wood RD. Coverley D, et al. Nucleic Acids Res. 1992 Aug 11;20(15):3873-80. doi: 10.1093/nar/20.15.3873. Nucleic Acids Res. 1992. PMID: 1508673 Free PMC article.
• Cytotoxicity of psammaplin A from a two-sponge association may correlate with the inhibition of DNA replication.
  Jiang Y, Ahn EY, Ryu SH, Kim DK, Park JS, Yoon HJ, You S, Lee BJ, Lee DS, Jung JH. Jiang Y, et al. BMC Cancer. 2004 Sep 30;4:70. doi: 10.1186/1471-2407-4-70. BMC Cancer. 2004. PMID: 15456519 Free PMC article.
• ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits.
  Cai J, Yao N, Gibbs E, Finkelstein J, Phillips B, O'Donnell M, Hurwitz J. Cai J, et al. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11607-12. doi: 10.1073/pnas.95.20.11607. Proc Natl Acad Sci U S A. 1998. PMID: 9751713 Free PMC article.
• Detecting the ability of viral, bacterial and eukaryotic replication proteins to track along DNA.
  Tinker RL, Kassavetis GA, Geiduschek EP. Tinker RL, et al. EMBO J. 1994 Nov 15;13(22):5330-7. doi: 10.1002/j.1460-2075.1994.tb06867.x. EMBO J. 1994. PMID: 7957099 Free PMC article.
• Identification and characterization of the fourth single-stranded-DNA binding domain of replication protein A.
  Brill SJ, Bastin-Shanower S. Brill SJ, et al. Mol Cell Biol. 1998 Dec;18(12):7225-34. doi: 10.1128/MCB.18.12.7225. Mol Cell Biol. 1998. PMID: 9819409 Free PMC article.

References

1. 1. Cell. 1988 Apr 8;53(1):117-26 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2523-7 - PubMed
3. 1. EMBO J. 1988 Apr;7(4):1211-8 - PubMed
4. 1. Science. 1988 Sep 16;241(4872):1486-9 - PubMed
5. 1. EMBO J. 1988 Oct;7(10):3149-58 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central