Exogenous and evoked oxytocin restores social behavior in the Cntnap2 mouse model of autism - PubMed (original) (raw)

Figure 1. Oxytocin administration rescues social behavior in the Cntnap2 mouse model

(A) Reciprocal social interaction test for WT (left) and KO (right) drug or vehicle treated mice by i.p. injection. The time spent engaged in social interaction for each pair of mice is shown (n=6 pairs, 3M-3F, per genotype/condition). Two-way ANOVA F11,60=2.89, p=0.004, treatment effect F5,60=4.13, p=0.0027, treatment/genotype interaction F5,60=2.23, p=0.0627, followed by Dunnett pairwise comparisons to controls WTvehicle-KOvehicle p=0.03, KOvehicle-KOOXT p=0.03, KOvehicle-KOAVP p=0.04. (B) Social approach (3 chamber) test. Time spent interacting with an empty cup or a cup with a stranger mouse inside is shown for each genotype when treated i.p. with vehicle, OXT or AVP (n=8–10, 4/5M-4/5F, mice per genotype/condition). Paired Student t test comparing 'mouse' to 'empty' as a measure of sociability within each group. WTvehicle p=0.01, WTOXT p=0.02, KOOXT=0.03**(C)** Reciprocal social interaction test when OXT and AVP are administered intranasally (n=8, 4M-4F, pairs per genotype/condition). Two-way ANOVA F5,42=15.88, p<0.0001, treatment effect F2,42=21.08, p<0.0001, genotype/treatment interaction F2,42=17.01, p≤0.0001 followed by Dunnett pairwise comparisons to controls WTvehicle-KOvehiclep<0.0001, KOvehicle-KOOXT p<0.0001, KOvehicle-KOAVP p<0.0001. **(D)**Reciprocal social interaction test (n=8 pairs of mice, 4M-4F, per genotype/condition). One-way ANOVA F5,41=22.02, p<0.0001 followed by Bonferroni post-hoc test, modified significance level p=0.003, all significant comparisons p<0.0001. (E) Reciprocal social interaction test showing time course of a single acute dose of intranasal OXT administration (n=8 pairs of mice, 4M-4F, per genotype/condition). One-way ANOVA F4,35=23.16, p<0.0001, followed by Bonferroni post-hoc test, modified significance level p=0.005, all significant comparisons p<0.0001. M=male, F=female. Box-plots represent the median plus the 25th and 75th percentiles. Whiskers represent the minimum and maximum values. The mean is represented as a plus (+) sign.