In vitro and in vivo identification of metabolites of magnoflorine by LC LTQ-Orbitrap MS and its potential pharmacokinetic interaction in Coptidis Rhizoma decoction in rat - PubMed (original) (raw)
. 2015 Aug;29(8):1235-48.
doi: 10.1002/bmc.3413. Epub 2015 Jan 22.
Affiliations
- PMID: 25611590
- DOI: 10.1002/bmc.3413
In vitro and in vivo identification of metabolites of magnoflorine by LC LTQ-Orbitrap MS and its potential pharmacokinetic interaction in Coptidis Rhizoma decoction in rat
Baojuan Xue et al. Biomed Chromatogr. 2015 Aug.
Abstract
Magnoflorine, an important aporphine alkaloid in Coptidis Rhizoma, is increasingly attracting research attention because of its pharmacological activities. The in vivo and in vitro metabolism of magnoflorine was investigated by LC LTQ-Orbitrap MS. In vivo samples including rat urine, feces, plasma and bile were collected separately after both oral (50 mg kg(-1) ) and intravenous administration (10 mg kg(-1) ) of magnoflorine, along with in vitro samples prepared by incubating magnoflorine with rat intestinal flora and liver microsome. As a result, 12 metabolites were found in biological samples. Phase I metabolites were identified in all biological samples, while phase II metabolites were mainly detected in urine, plasma and bile. In a pharmacokinetic study, rats were not only dosed with magnoflorine via oral (15, 30 and 60 mg kg(-1) ) and intravenous administration (10 mg kg(-1) ) but also dosed with Coptidis Rhizoma decoction (equivalent to 30 mg kg(-1) of magnoflorine) by intragastric administration to investigate the interaction of magnoflorine with the rest of compounds in Coptidis Rhizoma. Studies showed that magnoflorine possessed lower bioavailability and faster absorption and elimination. However, pharmacokinetic parameters altered significantly (p < 0.05) when magnoflorine was administered in Coptidis Rhizoma decoction. Oral gavage of Coptidis Rhizoma decoction decreased the absorption and elimination rates of magnoflorine, which revealed that there existed pharmacokinetic interactions between magnoflorine and the rest of ingredients in Coptidis Rhizoma.
Keywords: Coptidis Rhizoma decoction; LC LTQ-Orbitrap MS; magnoflorine; metabolites; pharmacokinetic interaction.
Copyright © 2015 John Wiley & Sons, Ltd.
Similar articles
- In Vitro Intestinal Absorption and Metabolism of Magnoflorine and its Potential Interaction in Coptidis Rhizoma Decoction in Rat.
Xue B, Zhao Y, Su J, Miao Q, Miao P, Chen N, Wang Z, Zhang Y, Ma S. Xue B, et al. Eur J Drug Metab Pharmacokinet. 2017 Apr;42(2):281-293. doi: 10.1007/s13318-016-0344-3. Eur J Drug Metab Pharmacokinet. 2017. PMID: 27209587 - [Influence of coexisting components in Coptidis Rhizoma on excretion of magnoflorine in rat bile, urine, and feces].
Xue BJ, Zhao WW, Sun XR, Zhang Z, Chen JH, Zhang YJ. Xue BJ, et al. Zhongguo Zhong Yao Za Zhi. 2022 Jul;47(14):3908-3914. doi: 10.19540/j.cnki.cjcmm.20220121.201. Zhongguo Zhong Yao Za Zhi. 2022. PMID: 35850849 Chinese. - Study on the PK profiles of magnoflorine and its potential interaction in Cortex phellodendri decoction by LC-MS/MS.
Tian X, Li Z, Lin Y, Chen M, Pan G, Huang C. Tian X, et al. Anal Bioanal Chem. 2014 Jan;406(3):841-9. doi: 10.1007/s00216-013-7530-9. Epub 2013 Dec 15. Anal Bioanal Chem. 2014. PMID: 24337185 - Rhizoma Coptidis for Alzheimer's Disease and Vascular Dementia: A Literature Review.
Wang Z, Yang Y, Liu M, Wei Y, Liu J, Pei H, Li H. Wang Z, et al. Curr Vasc Pharmacol. 2020;18(4):358-368. doi: 10.2174/1570161117666190710151545. Curr Vasc Pharmacol. 2020. PMID: 31291876 Review. - Magnoflorine: A review of its pharmacology, pharmacokinetics and toxicity.
Xu T, Kuang T, Du H, Li Q, Feng T, Zhang Y, Fan G. Xu T, et al. Pharmacol Res. 2020 Feb;152:104632. doi: 10.1016/j.phrs.2020.104632. Epub 2020 Jan 3. Pharmacol Res. 2020. PMID: 31911246 Review.
Cited by
- A Combination of Magnoflorine and Spinosin Improves the Antidepressant effects on CUMS Mouse Model.
Bi F, Wang Z, Guo Y, Xia M, Zhu X, Qiao W. Bi F, et al. Curr Drug Metab. 2024;25(1):71-80. doi: 10.2174/0113892002284230240213064248. Curr Drug Metab. 2024. PMID: 38415474 - Natural aporphine alkaloids: A comprehensive review of phytochemistry, pharmacokinetics, anticancer activities, and clinical application.
Sun J, Zhan X, Wang W, Yang X, Liu Y, Yang H, Deng J, Yang H. Sun J, et al. J Adv Res. 2024 Sep;63:231-253. doi: 10.1016/j.jare.2023.11.003. Epub 2023 Nov 5. J Adv Res. 2024. PMID: 37935346 Free PMC article. Review. - A Systematic Method for the Identification of Aporphine Alkaloid Constituents in Sabia schumanniana Diels Using UHPLC-Q-Exactive Orbitrap/Mass Spectrometry.
E S, Shang ZC, Qin SH, Li KL, Liu YN, Wu JL, Yan F, Cai W. E S, et al. Molecules. 2022 Nov 7;27(21):7643. doi: 10.3390/molecules27217643. Molecules. 2022. PMID: 36364479 Free PMC article. - Coptidis Rhizoma: a comprehensive review of its traditional uses, botany, phytochemistry, pharmacology and toxicology.
Wang J, Wang L, Lou GH, Zeng HR, Hu J, Huang QW, Peng W, Yang XB. Wang J, et al. Pharm Biol. 2019 Dec;57(1):193-225. doi: 10.1080/13880209.2019.1577466. Pharm Biol. 2019. PMID: 30963783 Free PMC article. Review. - Effect of Rhizoma coptidis (Huang Lian) on Treating Diabetes Mellitus.
Pang B, Yu XT, Zhou Q, Zhao TY, Wang H, Gu CJ, Tong XL. Pang B, et al. Evid Based Complement Alternat Med. 2015;2015:921416. doi: 10.1155/2015/921416. Epub 2015 Oct 5. Evid Based Complement Alternat Med. 2015. PMID: 26508987 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials