Dysbiosis of fungal microbiota in the intestinal mucosa of patients with colorectal adenomas - PubMed (original) (raw)

Clinical Trial

doi: 10.1038/srep07980.

Lingling Xie 2, Xi Yang 1, Huifang Miao 3, Na Lv 4, Ruifen Zhang 4, Xue Xiao 4, Yongfei Hu 4, Yulan Liu 2, Na Wu 2, Yuanmin Zhu 2, Baoli Zhu 5

Affiliations

Clinical Trial

Dysbiosis of fungal microbiota in the intestinal mucosa of patients with colorectal adenomas

Chunguang Luan et al. Sci Rep. 2015.

Abstract

The fungal microbiota is an important component of the human gut microbiome and may be linked to gastrointestinal disease. In this study, the fungal microbiota of biopsy samples from adenomas and adjacent tissues was characterized by deep sequencing. Ascomycota, Glomeromycota and Basidiomycota were identified as the dominant phyla in both adenomas and adjacent tissues from all subjects. Among the 60 genera identified, the opportunist pathogens Phoma and Candida represented an average of 45% of the fungal microbiota. When analyzed at the operational taxonomic unit (OTU) level, however, a decreased diversity in adenomas was observed, and three OTUs differed significantly from the adjacent tissues. Principal Component Analysis (PCA) revealed that the core OTUs formed separate clusters for advanced and non-advanced adenomas for which the abundance of four OTUs differed significantly. Moreover, the size of adenomas and the disease stage were closely related to changes in the fungal microbiota in subjects with adenomas. This study characterized the fungal microbiota profile of subjects with adenomas and identified potential diagnostic biomarkers closely related to different stages of adenomas.

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Figures

Figure 1

Figure 1. Distribution of fungi in adenomas and adjacent biopsy samples at the phylum (Figure 1A), genus (Figure 1B) and species (Figure 1C) levels.

A: Adenoma biopsy; N: Normal biopsy. 1–27: case number of the biopsy samples.

Figure 2

Figure 2

(Figure 2A). The OTU attribution in adenomas and adjacent mucosa biopsy samples. The blue circle indicates the OTUs present in adjacent biopsy samples, and the brown circle indicates the OTUs present in the adenoma biopsy samples; overlap indicates the OTU shared by the two types of biopsy samples. (Figure 2B). Rarefaction curve demonstrating fungal sequence coverage in each biopsy. The curve depicts the number of OTUs observed at different sequencing depths where the _x_-axis is the number of sequences and the _y_-axis is the number of OTUs observed in each biopsy.

Figure 3

Figure 3

(Figure 3A). The heatmap and hierarchical clustering of subjects with advanced and non-advanced adenoma biopsy samples based on the core OTUs. Blue circles indicate the advanced biopsy samples; gray circles indicate the non-advanced biopsy samples. (Figure 3B). PCA analysis on advanced and non-advanced adenoma biopsy samples based on core OTUs. Red circles indicate the advanced adenomas; blue circles indicate the non-advanced biopsy samples.

Figure 4

Figure 4

(Figure 4A). OTU 144089, which was assigned to phylum Basidiomycota, exhibited significant enrichment in adjacent tissues (p = 0.01) compared with adenoma biopsy samples in 27 paired biopsy samples. (Figure 4B). (OTU 697566), (4C) (OTU 144089) and (4D) (OTU 196869) demonstrate that the OTUs differed significantly between adenoma and adjacent biopsy samples in subjects with advanced and non-advanced disease. (Figure 4E). (OTU 5543) and (4F) (OTU 157510) demonstrate that the OTUs differed significantly in adenoma biopsy samples between subjects with advanced and non-advanced disease. (Figure 4G). (OTU 47507) and (4H) (OTU 339) indicate that the OTUs differed significantly in adjacent biopsy samples between subjects with advanced and non-advanced disease.

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