CD4+ lymphocyte function with early human immunodeficiency virus infection - PubMed (original) (raw)
CD4+ lymphocyte function with early human immunodeficiency virus infection
R J Gurley et al. Proc Natl Acad Sci U S A. 1989 Mar.
Abstract
The pathogenesis of cellular immune deficiency following human immunodeficiency virus (HIV) infection could result from quantitative and/or qualitative dysfunction of the CD4+ lymphocyte population. To better characterize the T-cell response to soluble antigen with HIV infection, we have isolated peripheral blood lymphocytes and purified populations of CD4+ lymphocytes from healthy HIV antibody-positive subjects, patients with acquired immunodeficiency syndrome (AIDS)-related complex (ARC), and healthy HIV antibody-negative controls. T-lymphocyte function was determined by proliferative response to lectin (phytohemagglutinin), phorbol 12-myristate 13-acetate (PMA), calcium ionophore, purified recombinant HIV envelope gp120, tetanus toxoid antigen, and tetanus toxoid antigen in the presence of recombinant gp120 or purified recombinant soluble CD4. PBLs and CD4+ lymphocytes from asymptomatic HIV-infected subjects responded equally well to lectin, PMA, and/or calcium ionophore and to tetanus toxoid as cells from uninfected control subjects. The cells that proliferated in response to a soluble antigenic stimulus did not respond to gp120. Cells from subjects with ARC had a selective antigen recognition defect independent of the number of CD4+ lymphocytes. Recombinant gp120 inhibited CD4+ lymphocyte proliferation to antigenic stimulus by 30-40%. Recombinant soluble CD4, a proposed therapeutic for HIV, had no effect on T-cell response to antigen. A selective antigen recognition response was not compromised early in HIV infection but was compromised in subjects with ARC. Inhibition of proliferation to tetanus toxoid by gp120 suggests that HIV may affect major histocompatibility complex II restricted antigen recognition independent of CD4+ cell loss.
Similar articles
- Detection of three distinct patterns of T helper cell dysfunction in asymptomatic, human immunodeficiency virus-seropositive patients. Independence of CD4+ cell numbers and clinical staging.
Clerici M, Stocks NI, Zajac RA, Boswell RN, Lucey DR, Via CS, Shearer GM. Clerici M, et al. J Clin Invest. 1989 Dec;84(6):1892-9. doi: 10.1172/JCI114376. J Clin Invest. 1989. PMID: 2574188 Free PMC article. - CD4+ lymphocyte function with early human immunodeficiency virus infection.
[No authors listed] [No authors listed] Dis Markers. 1990 Jan-Feb;8(1):40-1. Dis Markers. 1990. PMID: 1968803 No abstract available. - The CD4 antigen: physiological ligand and HIV receptor.
Sattentau QJ, Weiss RA. Sattentau QJ, et al. Cell. 1988 Mar 11;52(5):631-3. doi: 10.1016/0092-8674(88)90397-2. Cell. 1988. PMID: 2830988 Review. No abstract available. - Characterization of CD4 glycoprotein determinant-HIV envelope protein interactions: perspectives for analog and vaccine development.
Farrar WL, Harel-Bellan A, Ferris DK. Farrar WL, et al. Crit Rev Immunol. 1988;8(4):315-39. Crit Rev Immunol. 1988. PMID: 2850890 Review.
Cited by
- Performance verification of the new fully automated Aquios flow cytometer PanLeucogate (PLG) platform for CD4-T-lymphocyte enumeration in South Africa.
Coetzee LM, Glencross DK. Coetzee LM, et al. PLoS One. 2017 Nov 3;12(11):e0187456. doi: 10.1371/journal.pone.0187456. eCollection 2017. PLoS One. 2017. PMID: 29099874 Free PMC article. - Performance Evaluation of the Becton Dickinson FACSPresto™ Near-Patient CD4 Instrument in a Laboratory and Typical Field Clinic Setting in South Africa.
Coetzee LM, Moodley K, Glencross DK. Coetzee LM, et al. PLoS One. 2016 May 25;11(5):e0156266. doi: 10.1371/journal.pone.0156266. eCollection 2016. PLoS One. 2016. PMID: 27224025 Free PMC article. - Disulfide reduction in CD4 domain 1 or 2 is essential for interaction with HIV glycoprotein 120 (gp120), which impairs thioredoxin-driven CD4 dimerization.
Cerutti N, Killick M, Jugnarain V, Papathanasopoulos M, Capovilla A. Cerutti N, et al. J Biol Chem. 2014 Apr 11;289(15):10455-10465. doi: 10.1074/jbc.M113.539353. Epub 2014 Feb 18. J Biol Chem. 2014. PMID: 24550395 Free PMC article. - Chemokine coreceptor signaling in HIV-1 infection and pathogenesis.
Wu Y, Yoder A. Wu Y, et al. PLoS Pathog. 2009 Dec;5(12):e1000520. doi: 10.1371/journal.ppat.1000520. Epub 2009 Dec 24. PLoS Pathog. 2009. PMID: 20041213 Free PMC article. Review. - The co-receptor signaling model of HIV-1 pathogenesis in peripheral CD4 T cells.
Wu Y. Wu Y. Retrovirology. 2009 May 1;6:41. doi: 10.1186/1742-4690-6-41. Retrovirology. 2009. PMID: 19409100 Free PMC article.
References
- Nature. 1987 Aug 13-19;328(6131):626-9 - PubMed
- J Immunol. 1986 Oct 15;137(8):2514-21 - PubMed
- Science. 1988 Jan 15;239(4837):295-7 - PubMed
- Nature. 1988 Jan 7;331(6151):78-81 - PubMed
- Science. 1988 Feb 5;239(4840):617-22 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials