Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry - PubMed (original) (raw)

Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Shuping Nie et al. J Clin Microbiol. 2015 Apr.

Abstract

We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.

PubMed Disclaimer

Figures

FIG 1

Identification of F. nucleatum to subspecies level by MALDI-TOF MS. (A) MALDI-TOF mass spectra of five type strains of the F. nucleatum subspecies. The upper plot shows an enlargement (6,100 to 6,500 Da) of the full mass range (3,000 to 13,000 Da), highlighting differences in peak patterns for this range. ATCC 51191, subsp. animalis; ATCC 51190, subsp. fusiforme; ATCC 49256, subsp. vincentii; ATCC 10953, subsp. polymorphum; ATCC 25586, subsp. nucleatum. (B) Cluster analysis of mass spectra of type and clinical strains of four (five) Fusobacterium nucleatum subspecies used for the amendment of the reference database. For each strain, duplicate measurements were considered.

Cited by

A newly developed PCR-based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancer.
Bi D, Zhu Y, Gao Y, Li H, Zhu X, Wei R, Xie R, Wei Q, Qin H. Bi D, et al. Microb Biotechnol. 2021 Sep;14(5):2176-2186. doi: 10.1111/1751-7915.13900. Epub 2021 Jul 26. Microb Biotechnol. 2021. PMID: 34309194 Free PMC article.
What Do We See in Spectra?: Assignment of High-Intensity Peaks of Cutibacterium and Staphylococcus Spectra of MALDI-TOF Mass Spectrometry by Interspecies Comparative Proteogenomics.
Dekio I, Sugiura Y, Hamada-Tsutsumi S, Murakami Y, Tamura H, Suematsu M. Dekio I, et al. Microorganisms. 2021 Jun 8;9(6):1243. doi: 10.3390/microorganisms9061243. Microorganisms. 2021. PMID: 34201063 Free PMC article.
Detection of Fusobaterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis.
Janati AI, Karp I, Laprise C, Sabri H, Emami E. Janati AI, et al. Syst Rev. 2020 Dec 3;9(1):276. doi: 10.1186/s13643-020-01526-z. Syst Rev. 2020. PMID: 33272322 Free PMC article.
Analysis of 16S rRNA genes reveals reduced Fusobacterial community diversity when translocating from saliva to GI sites.
Richardson M, Ren J, Rubinstein MR, Taylor JA, Friedman RA, Shen B, Han YW. Richardson M, et al. Gut Microbes. 2020 Nov 9;12(1):1-13. doi: 10.1080/19490976.2020.1814120. Gut Microbes. 2020. PMID: 33054632 Free PMC article.
Sex-specific differences in the occurrence of Fusobacterium nucleatum subspecies and Fusobacterium periodonticum in the oral cavity.
Henne K, Schilling H, Stoneking M, Conrads G, Horz HP. Henne K, et al. Oncotarget. 2018 Apr 17;9(29):20631-20639. doi: 10.18632/oncotarget.25042. eCollection 2018 Apr 17. Oncotarget. 2018. PMID: 29755677 Free PMC article.

References

1. Allen-Vercoe E, Strauss J, Chadee K. 2011. Fusobacterium nucleatum: an emerging gut pathogen? Gut Microbes 2:294–298. doi:10.4161/gmic.2.5.18603. - DOI - PubMed
1. Han YW. 2015. Fusobacterium nucleatum: a commensal-turned pathogen. Curr Opin Microbiol 23C:141–147. doi:10.1016/j.mib.2014.11.013. - DOI - PMC - PubMed
1. Strauss J, White A, Ambrose C, McDonald J, Allen-Vercoe E. 2008. Phenotypic and genotypic analyses of clinical Fusobacterium nucleatum and Fusobacterium periodonticum isolates from the human gut. Anaerobe 14:301–309. doi:10.1016/j.anaerobe.2008.12.003. - DOI - PubMed
1. Kim HS, Lee DS, Chang YH, Kim MJ, Koh S, Kim J, Seong JH, Song SK, Shin HS, Son JB, Jung MY, Park SN, Yoo SY, Cho KW, Kim DK, Moon S, Kim D, Choi Y, Kim BO, Jang HS, Kim CS, Kim C, Choe SJ, Kook JK. 2010. Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies. J Clin Microbiol 48:545–553. doi:10.1128/JCM.01631-09. - DOI - PMC - PubMed
1. Kook JK, Park SN, Lim YK, Choi MH, Cho E, Kong SW, Shin Y, Paek J, Chang YH. 2013. Fusobacterium nucleatum subsp. fusiforme Gharbia and Shah 1992 is a later synonym of Fusobacterium nucleatum subsp. vincentii Dzink et al. 1990. Curr Microbiol 66:414–417. doi:10.1007/s00284-012-0289-y. - DOI - PubMed

Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry - PubMed (original) (raw)