Clonal hematopoiesis demonstrated by X-linked DNA polymorphisms after allogeneic bone marrow transplantation - PubMed (original) (raw)

Clonal hematopoiesis demonstrated by X-linked DNA polymorphisms after allogeneic bone marrow transplantation

A G Turhan et al. N Engl J Med. 1989.

Abstract

We analyzed DNA from peripheral-blood and marrow cells from 12 recipients of allogeneic bone marrow transplants to determine whether monoclonal but otherwise normal hematopoiesis occurs in such patients. All patients were being treated for various forms of leukemia or lymphoma. In 10 patients, granulocytes isolated from peripheral-blood samples obtained 28 to 159 days after transplantation were polyclonal. In some, circulating T cells were isolated and also found to be polyclonal. In contrast, two patients had donor-derived monoclonal or oligoclonal hematopoiesis after transplantation. In one, DNA from circulating mononuclear cells obtained 29 days after transplantation revealed a monoclonal pattern on analysis of a restriction-fragment-length polymorphism in the phosphoglycerate kinase gene. In the other, analysis of a restriction-fragment-length polymorphism in the hypoxanthine phosphoribosyltransferase gene suggested the presence of a dominant clone in the granulocytes sampled 36 days after transplantation. When the latter patient was reassessed on day 267, the same clone of donor hematopoietic cells was still predominant and was found to include circulating T cells as well as granulocytes. We conclude that monoclonal hematopoiesis of donor origin may be observed in recipients of allogeneic bone marrow transplants, indicating that stem cells in normal adult human marrow are able to repopulate both lymphoid and myeloid compartments after transplantation.

PubMed Disclaimer

Comment in

Clonal hematopoiesis after bone marrow transplantation.
Nash R, Storb R, Torok-Storb B, Neiman P. Nash R, et al. N Engl J Med. 1989 Dec 21;321(25):1758-9. N Engl J Med. 1989. PMID: 2627234 No abstract available.

Cited by

Retrovirus-mediated gene transfer to purified hemopoietic stem cells with long-term lympho-myelopoietic repopulating ability.
Szilvassy SJ, Fraser CC, Eaves CJ, Lansdorp PM, Eaves AC, Humphries RK. Szilvassy SJ, et al. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8798-802. doi: 10.1073/pnas.86.22.8798. Proc Natl Acad Sci U S A. 1989. PMID: 2573066 Free PMC article.
Efficient marking of human cells with rapid but transient repopulating activity in autografted recipients.
Glimm H, Schmidt M, Fischer M, Schwarzwaelder K, Wissler M, Klingenberg S, Prinz C, Waller CF, Lange W, Eaves CJ, von Kalle C. Glimm H, et al. Blood. 2005 Aug 1;106(3):893-8. doi: 10.1182/blood-2004-07-2859. Epub 2005 Apr 21. Blood. 2005. PMID: 15845903 Free PMC article.
Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium.
Petzer AL, Hogge DE, Landsdorp PM, Reid DS, Eaves CJ. Petzer AL, et al. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1470-4. doi: 10.1073/pnas.93.4.1470. Proc Natl Acad Sci U S A. 1996. PMID: 8643656 Free PMC article.
Proliferation of totipotent hematopoietic stem cells in vitro with retention of long-term competitive in vivo reconstituting ability.
Fraser CC, Szilvassy SJ, Eaves CJ, Humphries RK. Fraser CC, et al. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1968-72. doi: 10.1073/pnas.89.5.1968. Proc Natl Acad Sci U S A. 1992. PMID: 1311857 Free PMC article.
Evidence for the maintenance of hematopoiesis in a large animal by the sequential activation of stem-cell clones.
Abkowitz JL, Linenberger ML, Newton MA, Shelton GH, Ott RL, Guttorp P. Abkowitz JL, et al. Proc Natl Acad Sci U S A. 1990 Nov;87(22):9062-6. doi: 10.1073/pnas.87.22.9062. Proc Natl Acad Sci U S A. 1990. PMID: 2247481 Free PMC article.

Clonal hematopoiesis demonstrated by X-linked DNA polymorphisms after allogeneic bone marrow transplantation - PubMed (original) (raw)