CD14, TLR4 and TRAM Show Different Trafficking Dynamics During LPS Stimulation - PubMed (original) (raw)
doi: 10.1111/tra.12274. Epub 2015 Mar 24.
Affiliations
- PMID: 25707286
- DOI: 10.1111/tra.12274
Free article
CD14, TLR4 and TRAM Show Different Trafficking Dynamics During LPS Stimulation
Dionne C G Klein et al. Traffic. 2015 Jul.
Free article
Abstract
Toll-like receptor 4 (TLR4) is responsible for the immediate response to Gram-negative bacteria and signals via two main pathways by recruitment of distinct pairs of adaptor proteins. Mal-MyD88 [Mal (MyD88-adaptor-like) - MYD88 (Myeloid differentiation primary response gene (88))] is recruited to the plasma membrane to initiate the signaling cascade leading to production of pro-inflammatory cytokines while TRAM-TRIF [TRAM (TRIF-related adaptor molecule)-TRIF (TIR-domain-containing adapter-inducing interferon-β)] is recruited to early endosomes to initiate the subsequent production of type I interferons. We have investigated the dynamics of TLR4 and TRAM during lipopolysaccharide (LPS) stimulation. We found that LPS induced a CD14-dependent immobile fraction of TLR4 in the plasma membrane. Total internal reflection fluorescence microscopy (TIRF) revealed that LPS stimulation induced clustering of TLR4 into small punctate structures in the plasma membrane containing CD14/LPS and clathrin, both in HEK293 cells and the macrophage model cell line U373-CD14. These results suggest that laterally immobilized TLR4 receptor complexes are being formed and prepared for endocytosis. RAB11A was found to be involved in localizing TRAM to the endocytic recycling compartment (ERC) and to early sorting endosomes. Moreover, CD14/LPS but not TRAM was immobilized on RAB11A-positive endosomes, which indicates that TRAM and CD14/LPS can independently be recruited to endosomes.
Keywords: CD14; FRAP; MyD88; TIRF microscopy; TLR4; TRAM; confocal microscopy; diffusion coefficient; half-life; mobile fraction.
© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Similar articles
- CD14 dependence of TLR4 endocytosis and TRIF signaling displays ligand specificity and is dissociable in endotoxin tolerance.
Rajaiah R, Perkins DJ, Ireland DD, Vogel SN. Rajaiah R, et al. Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8391-6. doi: 10.1073/pnas.1424980112. Epub 2015 Jun 23. Proc Natl Acad Sci U S A. 2015. PMID: 26106158 Free PMC article. - TRAM is required for TLR2 endosomal signaling to type I IFN induction.
Stack J, Doyle SL, Connolly DJ, Reinert LS, O'Keeffe KM, McLoughlin RM, Paludan SR, Bowie AG. Stack J, et al. J Immunol. 2014 Dec 15;193(12):6090-102. doi: 10.4049/jimmunol.1401605. Epub 2014 Nov 10. J Immunol. 2014. PMID: 25385819 Free PMC article. - CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide.
Biedroń R, Peruń A, Józefowski S. Biedroń R, et al. PLoS One. 2016 Apr 13;11(4):e0153558. doi: 10.1371/journal.pone.0153558. eCollection 2016. PLoS One. 2016. PMID: 27073833 Free PMC article. - TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling.
Ciesielska A, Matyjek M, Kwiatkowska K. Ciesielska A, et al. Cell Mol Life Sci. 2021 Feb;78(4):1233-1261. doi: 10.1007/s00018-020-03656-y. Epub 2020 Oct 15. Cell Mol Life Sci. 2021. PMID: 33057840 Free PMC article. Review. - Functional interfaces between TICAM-2/TRAM and TICAM-1/TRIF in TLR4 signaling.
Funami K, Matsumoto M, Oshiumi H, Inagaki F, Seya T. Funami K, et al. Biochem Soc Trans. 2017 Aug 15;45(4):929-935. doi: 10.1042/BST20160259. Epub 2017 Jun 19. Biochem Soc Trans. 2017. PMID: 28630139 Review.
Cited by
- Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer.
Pu X, Dong C, Zhu W, Li W, Jiang H. Pu X, et al. Onco Targets Ther. 2019 Sep 9;12:7361-7373. doi: 10.2147/OTT.S215131. eCollection 2019. Onco Targets Ther. 2019. PMID: 31571899 Free PMC article. - Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney.
Harada F, Uehara O, Morikawa T, Hiraki D, Onishi A, Toraya S, Adhikari BR, Takai R, Yoshida K, Sato J, Nishimura M, Chiba I, Wu CZ, Abiko Y. Harada F, et al. Med Mol Morphol. 2018 Sep;51(3):156-165. doi: 10.1007/s00795-018-0181-3. Epub 2018 Jan 31. Med Mol Morphol. 2018. PMID: 29388058 - A mathematical model relates intracellular TLR4 oscillations to sepsis progression.
Stan RC, Soriano FG, de Camargo MM. Stan RC, et al. BMC Res Notes. 2018 Jul 11;11(1):462. doi: 10.1186/s13104-018-3561-9. BMC Res Notes. 2018. PMID: 29996939 Free PMC article. - SLAMF1 is required for TLR4-mediated TRAM-TRIF-dependent signaling in human macrophages.
Yurchenko M, Skjesol A, Ryan L, Richard GM, Kandasamy RK, Wang N, Terhorst C, Husebye H, Espevik T. Yurchenko M, et al. J Cell Biol. 2018 Apr 2;217(4):1411-1429. doi: 10.1083/jcb.201707027. Epub 2018 Feb 12. J Cell Biol. 2018. PMID: 29440514 Free PMC article. - Review of research progress on intestinal microbiota based on metabolism and inflammation for depression.
Tan Y, Xu M, Lin D. Tan Y, et al. Arch Microbiol. 2024 Mar 10;206(4):146. doi: 10.1007/s00203-024-03866-z. Arch Microbiol. 2024. PMID: 38462572 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous