Glutamine synthetase II in Rhizobium: reexamination of the proposed horizontal transfer of DNA from eukaryotes to prokaryotes - PubMed (original) (raw)

Comparative Study

doi: 10.1007/BF02602912.

Affiliations

• PMID: 2575672
• DOI: 10.1007/BF02602912

Comparative Study

Glutamine synthetase II in Rhizobium: reexamination of the proposed horizontal transfer of DNA from eukaryotes to prokaryotes

R G Shatters et al. J Mol Evol. 1989 Nov.

Abstract

We have determined the DNA sequence of a Rhizobium meliloti gene that encodes glutamine synthetase II (GSII). The deduced amino acid sequence was compared to that of Bradyrhizobium japonicum GSII and those of various plant and mammalian glutamine synthetases (GS) in order to evaluate a proposal that the gene for this enzyme was recently transferred from plants to their symbiotic bacteria. There is 83.6% identity between the R. meliloti and B. japonicum proteins. The bacterial GSII proteins average 42.5% identity with the plant GS proteins and 41.8% identity with their mammalian counterparts. The plant proteins average 53.7% identity with the mammalian proteins. Thus, the GS proteins are highly conserved and the divergence of these proteins is proportional to the phylogenetic divergence of the organisms from which the sequences were determined. No transfer of genes across large taxonomic gaps is needed to explain the presence of GSII in these bacteria.

PubMed Disclaimer

Similar articles

• Molecular genetics of the glutamine synthetases in Rhizobium species.
  Espín G, Moreno S, Guzman J. Espín G, et al. Crit Rev Microbiol. 1994;20(2):117-23. doi: 10.3109/10408419409113551. Crit Rev Microbiol. 1994. PMID: 7915906 Review.
• The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications.
  Turner SL, Young JP. Turner SL, et al. Mol Biol Evol. 2000 Feb;17(2):309-19. doi: 10.1093/oxfordjournals.molbev.a026311. Mol Biol Evol. 2000. PMID: 10677854
• Regulation of glutamine synthetase II activity in Rhizobium meliloti 104A14.
  Shatters RG, Somerville JE, Kahn ML. Shatters RG, et al. J Bacteriol. 1989 Sep;171(9):5087-94. doi: 10.1128/jb.171.9.5087-5094.1989. J Bacteriol. 1989. PMID: 2570059 Free PMC article.
• Rhizobium meliloti 1021 has three differentially regulated loci involved in glutamine biosynthesis, none of which is essential for symbiotic nitrogen fixation.
  de Bruijn FJ, Rossbach S, Schneider M, Ratet P, Messmer S, Szeto WW, Ausubel FM, Schell J. de Bruijn FJ, et al. J Bacteriol. 1989 Mar;171(3):1673-82. doi: 10.1128/jb.171.3.1673-1682.1989. J Bacteriol. 1989. PMID: 2563998 Free PMC article.
• Evolution of glutamine synthetase genes is in accordance with the neutral theory of molecular evolution.
  Tateno Y. Tateno Y. Jpn J Genet. 1994 Oct;69(5):489-502. doi: 10.1266/jjg.69.489. Jpn J Genet. 1994. PMID: 7999370 Review.

Cited by

• Molecular cloning, sequencing, and expression of the glutamine synthetase II (glnII) gene from the actinomycete root nodule symbiont Frankia sp. strain CpI1.
  Rochefort DA, Benson DR. Rochefort DA, et al. J Bacteriol. 1990 Sep;172(9):5335-42. doi: 10.1128/jb.172.9.5335-5342.1990. J Bacteriol. 1990. PMID: 1975584 Free PMC article.
• Evolutionary relationships of bacterial and archaeal glutamine synthetase genes.
  Brown JR, Masuchi Y, Robb FT, Doolittle WF. Brown JR, et al. J Mol Evol. 1994 Jun;38(6):566-76. doi: 10.1007/BF00175876. J Mol Evol. 1994. PMID: 7916055
• Archaea and the prokaryote-to-eukaryote transition.
  Brown JR, Doolittle WF. Brown JR, et al. Microbiol Mol Biol Rev. 1997 Dec;61(4):456-502. doi: 10.1128/mmbr.61.4.456-502.1997. Microbiol Mol Biol Rev. 1997. PMID: 9409149 Free PMC article. Review.
• Genetic, molecular and developmental analysis of the glutamine synthetase isozymes of Drosophila melanogaster.
  Caggese C, Barsanti P, Viggiano L, Bozzetti MP, Caizzi R. Caggese C, et al. Genetica. 1994;94(2-3):275-81. doi: 10.1007/BF01443441. Genetica. 1994. PMID: 7896146 Review.

References

1. 1. J Bacteriol. 1984 Jun;158(3):1005-11 - PubMed
2. 1. J Bacteriol. 1983 Oct;156(1):168-76 - PubMed
3. 1. Nat New Biol. 1973 Jun 13;243(128):199-200 - PubMed
4. 1. J Biol Chem. 1986 Aug 15;261(23):10587-91 - PubMed
5. 1. J Bacteriol. 1980 Feb;141(2):996-8 - PubMed

Publication types

MeSH terms

Substances