The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence - PubMed (original) (raw)
Comparative Study
. 1985 May 25;260(10):6264-72.
- PMID: 2581950
Free article
Comparative Study
The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence
H E Schnepf et al. J Biol Chem. 1985.
Free article
Abstract
We have determined the nucleotide sequence of a 4222-base segment of DNA which contains the promoter, the coding region, and the terminator of a crystal protein gene cloned from a Bacillus thuringiensis plasmid. A sequence of 1176 amino acids encoding a Mr 133,500 peptide was deduced from the single open reading frame. This protein-coding region was analyzed for codon usage, predicted hydropathy, and predicted secondary structure. Examination of the base sequence revealed the presence of several inverted and direct repeats located in both the coding and noncoding regions. S1 nuclease mapping was used to locate the transcription termination point at a site following a potentially very stable stem-and-loop structure.
Similar articles
- Insecticidal crystal proteins of Bacillus thuringiensis.
Höfte H, Whiteley HR. Höfte H, et al. Microbiol Rev. 1989 Jun;53(2):242-55. doi: 10.1128/mr.53.2.242-255.1989. Microbiol Rev. 1989. PMID: 2666844 Free PMC article. Review. - Molecular characterization of a gene encoding a 72-kilodalton mosquito-toxic crystal protein from Bacillus thuringiensis subsp. israelensis.
Donovan WP, Dankocsik C, Gilbert MP. Donovan WP, et al. J Bacteriol. 1988 Oct;170(10):4732-8. doi: 10.1128/jb.170.10.4732-4738.1988. J Bacteriol. 1988. PMID: 2902069 Free PMC article. - Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis.
Waalwijk C, Dullemans AM, van Workum ME, Visser B. Waalwijk C, et al. Nucleic Acids Res. 1985 Nov 25;13(22):8207-17. doi: 10.1093/nar/13.22.8207. Nucleic Acids Res. 1985. PMID: 4070003 Free PMC article. - Genetic manipulation of Bacillus thuringiensis insecticidal crystal protein genes in bacteria.
Gawron-Burke C, Baum JA. Gawron-Burke C, et al. Genet Eng (N Y). 1991;13:237-63. doi: 10.1007/978-1-4615-3760-1_11. Genet Eng (N Y). 1991. PMID: 1367416 Review. No abstract available.
Cited by
- Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action.
Vílchez S. Vílchez S. Toxins (Basel). 2020 Sep 16;12(9):600. doi: 10.3390/toxins12090600. Toxins (Basel). 2020. PMID: 32948025 Free PMC article. Review. - Insecticidal crystal proteins of Bacillus thuringiensis.
Höfte H, Whiteley HR. Höfte H, et al. Microbiol Rev. 1989 Jun;53(2):242-55. doi: 10.1128/mr.53.2.242-255.1989. Microbiol Rev. 1989. PMID: 2666844 Free PMC article. Review. - Expression of the cryIB crystal protein gene of Bacillus thuringiensis.
Brizzard BL, Schnepf HE, Kronstad JW. Brizzard BL, et al. Mol Gen Genet. 1991 Dec;231(1):59-64. doi: 10.1007/BF00293822. Mol Gen Genet. 1991. PMID: 1840641 - Molecular characterization of a gene encoding a 72-kilodalton mosquito-toxic crystal protein from Bacillus thuringiensis subsp. israelensis.
Donovan WP, Dankocsik C, Gilbert MP. Donovan WP, et al. J Bacteriol. 1988 Oct;170(10):4732-8. doi: 10.1128/jb.170.10.4732-4738.1988. J Bacteriol. 1988. PMID: 2902069 Free PMC article. - Influence of transcriptional and translational control sequences on the expression of foreign genes in Caulobacter crescentus.
Yap WH, Thanabalu T, Porter AG. Yap WH, et al. J Bacteriol. 1994 May;176(9):2603-10. doi: 10.1128/jb.176.9.2603-2610.1994. J Bacteriol. 1994. PMID: 8169208 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources