Outer plaque assembly and spore encapsulation are defective during sporulation of adenylate cyclase-deficient mutants of Saccharomyces cerevisiae - PubMed (original) (raw)

Outer plaque assembly and spore encapsulation are defective during sporulation of adenylate cyclase-deficient mutants of Saccharomyces cerevisiae

I Uno et al. J Cell Biol. 1985 Jun.

Abstract

Sporulation in diploid cells homozygous for the cyr1-2 mutation of the yeast Saccharomyces cerevisiae was examined. This mutation causes a defect in adenylate cyclase and temperature-sensitive arrest in the G1 phase of the mitotic cell cycle. The cyr1-2/cyr1-2 diploid cells were able to initiate meiotic divisions, but produced predominantly two-spored asci at the restrictive temperature. Temperature-sensitive period for production of two-spored asci was approximately 12 h after the transfer of cells to the sporulation medium. The levels of cAMP increased during this period in the wild type and cyr1-2/cyr1-2 diploid cells incubated at the permissive temperature, but remained at an extremely low level in the cyr1-2/cyr1-2 diploid cells incubated at the restrictive temperature. Dyad analysis of the cyr1-2 strain indicated that meiotic products were randomly included into ascospores. Fluorescent microscopy of the cyr1-2/cyr1-2 diploid cells incubated at the restrictive temperature revealed that individual haploid nuclei were enclosed in each of the two spores after meiosis. About half of the cyr1-2/cyr1-2 diploid cells entered normal meiosis 1 producing two normal spindle pole bodies with inner and outer plaques, and the other half entered abnormal meiosis 1 producing one normal spindle pole body and one defective spindle pole body without out plaque. At meiosis II, some cells contained a pair of normal spindle pole bodies and other cells contained pairs of normal and abnormal spindle pole bodies.

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References

1. 1. Adv Cyclic Nucleotide Res. 1972;2:25-40 - PubMed
2. 1. Can J Microbiol. 1972 Dec;18(12):1897-905 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5056-60 - PubMed
4. 1. Experientia. 1981;37(9):969-71 - PubMed
5. 1. Genetics. 1981 Oct;99(2):197-209 - PubMed

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