A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis - PubMed (original) (raw)

PMID: 2582032

A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis

A A Vandenbark et al. J Immunol. 1985 Jul.

Abstract

A T lymphocyte line, BP-1, expressing the T helper phenotype was selected from Lewis rats immunized with guinea pig myelin basic protein (GP-BP) in complete Freund's adjuvant (CFA). The BP-1 line responded specifically to GP-BP but not to PPD after the first round of selection, and responded to rat but not human or bovine BP. When injected i.p. into histocompatible Lewis or F1 (Lewis X P2) recipients, the BP-1 line induced both clinical signs of experimental autoimmune encephalomyelitis (EAE) and delayed type hypersensitivity (DTH) reactions in ears challenged intradermally with GP-BP but not PPD. The severity of clinical signs and the degree of ear swelling were dependent on the dose of BP-1 cells injected. Both activities were detectable with as few as 0.1 X 10(6) BP-1 line cells and required prior activation of the line cells with GP-BP presented by accessory cells. Lewis rats that had recovered from EAE induced by injection of GP-BP in CFA were more susceptible than naive rats to BP-1 line-mediated disease, requiring as few as 0.03 X 10(6) line cells. Clinical EAE and DTH could be serially transferred into F1 (Lewis X P2) recipients with BP-1 cells and back to nonirradiated Lewis parents with activated splenocytes, suggesting that BP-1 cells persist in recipient rats. These results demonstrate the potent biologic activities of an autoreactive BP-specific T lymphocyte line. This line possesses properties similar to BP lines described previously as well as to culture-conditioned splenic T effector cells; thus, the data presented here bridge the gap between these two approaches for studying T effector lymphocyte functions.

PubMed Disclaimer

Cited by

Modulating co-stimulation.
Viglietta V, Khoury SJ. Viglietta V, et al. Neurotherapeutics. 2007 Oct;4(4):666-75. doi: 10.1016/j.nurt.2007.07.006. Neurotherapeutics. 2007. PMID: 17920548 Free PMC article. Review.
Hemorrhagic autoimmune encephalomyelitis induced by adoptive transfer of activated semiallogeneic spleen cells into irradiated rats.
Matsumoto Y, Kawai K, Fujiwara M. Matsumoto Y, et al. Am J Pathol. 1988 Nov;133(2):306-15. Am J Pathol. 1988. PMID: 2461090 Free PMC article.
Preferential T-cell receptor beta-chain variable gene use in myelin basic protein-reactive T-cell clones from patients with multiple sclerosis.
Kotzin BL, Karuturi S, Chou YK, Lafferty J, Forrester JM, Better M, Nedwin GE, Offner H, Vandenbark AA. Kotzin BL, et al. Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9161-5. doi: 10.1073/pnas.88.20.9161. Proc Natl Acad Sci U S A. 1991. PMID: 1717998 Free PMC article.
Molecular mimicry: can epitope mimicry induce autoimmune disease?
Davies JM. Davies JM. Immunol Cell Biol. 1997 Apr;75(2):113-26. doi: 10.1038/icb.1997.16. Immunol Cell Biol. 1997. PMID: 9107563 Free PMC article. Review.

A myelin basic protein-specific T lymphocyte line that mediates experimental autoimmune encephalomyelitis - PubMed (original) (raw)