A stable alpha-helical element in the carboxy-terminal domain of free and chromatin-bound histone H1 from sea urchin sperm - PubMed (original) (raw)
A stable alpha-helical element in the carboxy-terminal domain of free and chromatin-bound histone H1 from sea urchin sperm
C S Hill et al. EMBO J. 1989 Sep.
Abstract
The carboxy-terminal domain (residues 121-248) of sea urchin sperm-specific H1 is not random coil but partly alpha-helical, even in 1 mM sodium phosphate, pH 7. The helix resides in a 57 residue proline-free segment which, in the intact histone, immediately abuts the central globular domain. The proline-free region, which is rich in lysine and alanine, is relatively resistant to tryptic digestion when the carboxy-terminal domain is bound to DNA. Two (overlapping) resistant peptides are shown by circular dichroism measurements to be substantially alpha-helical in 1 mM sodium phosphate and to increase in helix content to approximately 70% in 1 M NaCLO4. Tryptic digestion of chromatin gives resistant fragments containing both the globular domain and the contiguous proline-free segment, strongly suggesting that the alpha-helical segment also exists in chromatin, where it would be ideally placed to direct the path of the linker DNA entering or leaving the nucleosome. The linker in sea urchin sperm chromatin is long (approximately 74 bp), and the unusually long alpha-helical segment in the carboxy-terminal tail of sperm H1 which has amphipathic character due to the alanine distribution, and is likely to be curved, may be a special feature tailored to organize it.
Similar articles
- The structure of sea-urchin-sperm histone phi 1 (H1) in chromatin and in free solution. Trypsin digestion and spectroscopic studies.
Puigdomenech P, Palau J, Crane-Robinson C. Puigdomenech P, et al. Eur J Biochem. 1980 Feb;104(1):263-70. doi: 10.1111/j.1432-1033.1980.tb04424.x. Eur J Biochem. 1980. PMID: 6768551 - Core histone-DNA interactions in sea urchin sperm chromatin. The N-terminal tail of H2B interacts with linker DNA.
Hill CS, Thomas JO. Hill CS, et al. Eur J Biochem. 1990 Jan 12;187(1):145-53. doi: 10.1111/j.1432-1033.1990.tb15288.x. Eur J Biochem. 1990. PMID: 2298202 - The chromatin of sea urchin sperm.
Puigdomenech P, Romero MC, Allan J, Sautière P, Giancotti V, Crane-Robinson C. Puigdomenech P, et al. Biochim Biophys Acta. 1987 Jan 28;908(1):70-80. doi: 10.1016/0167-4781(87)90023-6. Biochim Biophys Acta. 1987. PMID: 3801486 - Origin of H1 linker histones.
Kasinsky HE, Lewis JD, Dacks JB, Ausió J. Kasinsky HE, et al. FASEB J. 2001 Jan;15(1):34-42. doi: 10.1096/fj.00-0237rev. FASEB J. 2001. PMID: 11149891 Review. - Packaging and unpackaging the sea urchin sperm genome.
Poccia DL, Green GR. Poccia DL, et al. Trends Biochem Sci. 1992 Jun;17(6):223-7. doi: 10.1016/0968-0004(92)90382-j. Trends Biochem Sci. 1992. PMID: 1502725 Free PMC article. Review.
Cited by
- MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes.
Shen CH, Allan J. Shen CH, et al. Cells. 2021 Aug 29;10(9):2239. doi: 10.3390/cells10092239. Cells. 2021. PMID: 34571888 Free PMC article. - What is the role of histone H1 heterogeneity? A functional model emerges from a 50 year mystery.
Parseghian MH. Parseghian MH. AIMS Biophys. 2015;2(4):724-772. doi: 10.3934/biophy.2015.4.724. Epub 2015 Nov 16. AIMS Biophys. 2015. PMID: 31289748 Free PMC article. - DNA and nucleosomes direct distinct folding of a linker histone H1 C-terminal domain.
Fang H, Clark DJ, Hayes JJ. Fang H, et al. Nucleic Acids Res. 2012 Feb;40(4):1475-84. doi: 10.1093/nar/gkr866. Epub 2011 Oct 22. Nucleic Acids Res. 2012. PMID: 22021384 Free PMC article. - Projected [(1)H, (15)N]-HMQC-[ (1)H, (1)H]-NOESY for large molecular systems: application to a 121 kDa protein-DNA complex.
Galius V, Leontiou C, Richmond T, Wider G. Galius V, et al. J Biomol NMR. 2008 Mar;40(3):175-81. doi: 10.1007/s10858-008-9219-5. Epub 2008 Jan 30. J Biomol NMR. 2008. PMID: 18231863 - A variable topology for the 30-nm chromatin fibre.
Wu C, Bassett A, Travers A. Wu C, et al. EMBO Rep. 2007 Dec;8(12):1129-34. doi: 10.1038/sj.embor.7401115. EMBO Rep. 2007. PMID: 18059311 Free PMC article. Review.
References
- Biopolymers. 1981 Dec;20(12):2533-52 - PubMed
- Biochem J. 1981 Sep 1;197(3):655-60 - PubMed
- J Theor Biol. 1982 Jun 7;96(3):327-36 - PubMed
- Nature. 1983 Apr 7;302(5908):548-50 - PubMed
- Nature. 1983 Nov 17-23;306(5940):281-3 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous