G&T-seq: parallel sequencing of single-cell genomes and transcriptomes - PubMed (original) (raw)

doi: 10.1038/nmeth.3370. Epub 2015 Apr 27.

Wilfried Haerty 2, Parveen Kumar 3, Yang I Li 2, Tim Xiaoming Hu 2, Mabel J Teng 4, Mubeen Goolam 5, Nathalie Saurat 6, Paul Coupland 7, Lesley M Shirley 7, Miriam Smith 7, Niels Van der Aa 3, Ruby Banerjee 8, Peter D Ellis 7, Michael A Quail 7, Harold P Swerdlow 7, Magdalena Zernicka-Goetz 5, Frederick J Livesey 6, Chris P Ponting 9, Thierry Voet 10

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G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C Macaulay et al. Nat Methods. 2015 Jun.

Abstract

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

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