Antibody-forming cell induction during an early phase of germinal centre development and its delay with ageing - PubMed (original) (raw)
Antibody-forming cell induction during an early phase of germinal centre development and its delay with ageing
M H Kosco et al. Immunology. 1989 Nov.
Abstract
The present study was initiated to determine if an early phase of germinal centre (GC) development is associated with the generation of antibody-forming cells (AFC). Germinal centres in draining lymph nodes from immune mice were examined histochemically after secondary immunization for the presence of AFC at both the light and electron microscopic levels. Additionally, peanut agglutinin (PNA) high (Hi) GC B cells were isolated, placed in cell culture and specific antibody production was monitored at successive intervals. Electron microscopy showed that plasma cells in all stages of differentiation were present within GC at 3-5 days and to a lesser extent at 7 days following antigenic challenge. Furthermore, PNAHi GC B cells obtained between Days 3 and 5 spontaneously produced specific IgG when placed in culture. Germinal centre B cells isolated either before or after this period did not produce antibody without the addition of T-cell cytokines. Induction of AFC in GC occurred at the time when GC B cells acquire follicular dendritic cell (FDC)-derived, immune complex-coated bodies (iccosomes) and process and present this antigen to helper T cells. This suggested a causal relationship between iccosome release and AFC induction. Support for this was obtained by examination of AFC induction in aged mice where iccosome release has not been observed. Peanut agglutinin-positive GC B cells isolated from aged mice on Days 3-5 after antigen challenge failed to spontaneously produce specific antibody. Collectively, these data show that GC development 3-5 days after booster immunization results in AFC generation and suggests a role for FDC iccosomes in their induction.
Similar articles
- Follicular dendritic cell-derived antigen and accessory activity in initiation of memory IgG responses in vitro.
Wu J, Qin D, Burton GF, Szakal AK, Tew JG. Wu J, et al. J Immunol. 1996 Oct 15;157(8):3404-11. J Immunol. 1996. PMID: 8871638 - In vivo obtained antigen presented by germinal center B cells to T cells in vitro.
Kosco MH, Szakal AK, Tew JG. Kosco MH, et al. J Immunol. 1988 Jan 15;140(2):354-60. J Immunol. 1988. PMID: 3257234 - Development of immuneeoglobulin and antibody-forming cells in different stages of the immun response.
Avrameas S, Antoine JC, Ternynck T, Petit C. Avrameas S, et al. Ann Immunol (Paris). 1976 Jun-Jul;127(3-4):551-71. Ann Immunol (Paris). 1976. PMID: 60908 Review.
Cited by
- Memory B cell development elicited by mRNA booster vaccinations in the elderly.
Wang Z, Muecksch F, Raspe R, Johannsen F, Turroja M, Canis M, ElTanbouly MA, Santos GSS, Johnson B, Baharani VA, Patejak R, Yao KH, Chirco BJ, Millard KG, Shimeliovich I, Gazumyan A, Oliveira TY, Bieniasz PD, Hatziioannou T, Caskey M, Nussenzweig MC. Wang Z, et al. J Exp Med. 2023 Sep 4;220(9):e20230668. doi: 10.1084/jem.20230668. Epub 2023 Jun 27. J Exp Med. 2023. PMID: 37368240 Free PMC article. - Spatial dysregulation of T follicular helper cells impairs vaccine responses in aging.
Silva-Cayetano A, Fra-Bido S, Robert PA, Innocentin S, Burton AR, Watson EM, Lee JL, Webb LMC, Foster WS, McKenzie RCJ, Bignon A, Vanderleyden I, Alterauge D, Lemos JP, Carr EJ, Hill DL, Cinti I, Balabanian K, Baumjohann D, Espeli M, Meyer-Hermann M, Denton AE, Linterman MA. Silva-Cayetano A, et al. Nat Immunol. 2023 Jul;24(7):1124-1137. doi: 10.1038/s41590-023-01519-9. Epub 2023 May 22. Nat Immunol. 2023. PMID: 37217705 Free PMC article. - Aging-Related Cellular, Structural and Functional Changes in the Lymph Nodes: A Significant Component of Immunosenescence? An Overview.
Cakala-Jakimowicz M, Kolodziej-Wojnar P, Puzianowska-Kuznicka M. Cakala-Jakimowicz M, et al. Cells. 2021 Nov 12;10(11):3148. doi: 10.3390/cells10113148. Cells. 2021. PMID: 34831371 Free PMC article. Review. - Aging and Options to Halt Declining Immunity to Virus Infections.
Palacios-Pedrero MÁ, Osterhaus ADME, Becker T, Elbahesh H, Rimmelzwaan GF, Saletti G. Palacios-Pedrero MÁ, et al. Front Immunol. 2021 May 12;12:681449. doi: 10.3389/fimmu.2021.681449. eCollection 2021. Front Immunol. 2021. PMID: 34054872 Free PMC article. Review. - Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination.
Stebegg M, Bignon A, Hill DL, Silva-Cayetano A, Krueger C, Vanderleyden I, Innocentin S, Boon L, Wang J, Zand MS, Dooley J, Clark J, Liston A, Carr E, Linterman MA. Stebegg M, et al. Elife. 2020 Mar 24;9:e52473. doi: 10.7554/eLife.52473. Elife. 2020. PMID: 32204792 Free PMC article.
References
- J Immunol. 1983 Nov;131(5):2254-7 - PubMed
- J Immunol. 1983 Oct;131(4):1714-27 - PubMed
- Infect Immun. 1985 Sep;49(3):487-93 - PubMed
- J Leukoc Biol. 1986 Aug;40(2):147-57 - PubMed
- J Immunol. 1988 Jan 15;140(2):341-53 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous