A household serosurvey to estimate the magnitude of a dengue outbreak in Mombasa, Kenya, 2013 - PubMed (original) (raw)

. 2015 Apr 29;9(4):e0003733.

doi: 10.1371/journal.pntd.0003733. eCollection 2015 Apr.

John C Neatherlin 2, Mark Delorey 3, Melvin Ochieng 2, Abdinoor Haji Mohamed 4, Daniel Ondari Mogeni 4, Elizabeth Hunsperger 1, Shem Patta 5, Stella Gikunju 3, Lilian Waiboic 2, Barry Fields 2, Victor Ofula 6, Samson Limbaso Konongoi 6, Brenda Torres-Velasquez 1, Nina Marano 2, Rosemary Sang 6, Harold S Margolis 1, Joel M Montgomery 2, Kay M Tomashek 1

Affiliations

A household serosurvey to estimate the magnitude of a dengue outbreak in Mombasa, Kenya, 2013

Esther M Ellis et al. PLoS Negl Trop Dis. 2015.

Abstract

Dengue appears to be endemic in Africa with a number of reported outbreaks. In February 2013, several individuals with dengue-like illnesses and negative malaria blood smears were identified in Mombasa, Kenya. Dengue was laboratory confirmed and an investigation was conducted to estimate the magnitude of local transmission including a serologic survey to determine incident dengue virus (DENV) infections. Consenting household members provided serum and were questioned regarding exposures and medical history. RT-PCR was used to identify current DENV infections and IgM anti-DENV ELISA to identify recent infections. Of 1,500 participants from 701 households, 210 (13%) had evidence of current or recent DENV infection. Among those infected, 93 (44%) reported fever in the past month. Most (68, 73%) febrile infected participants were seen by a clinician and all but one of 32 participants who reportedly received a diagnosis were clinically diagnosed as having malaria. Having open windows at night (OR = 2.3; CI: 1.1-4.8), not using daily mosquito repellent (OR = 1.6; CI: 1.0-2.8), and recent travel outside of Kenya (OR = 2.5; CI: 1.1-5.4) were associated with increased risk of DENV infection. This survey provided a robust measure of incident DENV infections in a setting where cases were often unrecognized and misdiagnosed.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1

Fig 1. Epidemiological curve of 267 suspected dengue cases detected at hospitals from January–May, 2013, of which 155 (58%) were confirmed to have a current DENV infection and included a single fatal case.

Fig 2

Fig 2. Map of 210 dengue virus (DENV) infected participants in Tudor, Mombasa, Kenya.

Participants with evidence of current or recent DENV infection were distributed throughout the Tudor district, and there was no statistically significant clustering by area. Solid black circles represent IgM anti-DENV positive participants, white circles represent RT-PCR positive participants.

References

    1. Gubler DJ, Clark GG (1995) Dengue/dengue hemorrhagic fever: the emergence of a global health problem. Emerg Infect Dis 1: 55–57. -PMC -PubMed
    1. Gubler DJ (2004) The changing epidemiology of yellow fever and dengue, 1900 to 2003: full circle? Comp Immunol Microbiol Infect Dis 27: 319–330. -PubMed
    1. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, et al. (2013) The global distribution and burden of dengue. Nature 496: 504–507. 10.1038/nature12060 -DOI -PMC -PubMed
    1. (2009). Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition. Geneva: -PubMed
    1. Vazquez S, Ruiz D, Barrero R, Ramirez R, Calzada N, et al. (2010) Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients. Diagn Microbiol Infect Dis 68: 46–49. 10.1016/j.diagmicrobio.2010.04.006 -DOI -PubMed

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