In vitro evidence that Langerhans cells can adopt two functionally distinct forms capable of antigen presentation to T lymphocytes - PubMed (original) (raw)
Comparative Study
. 1989 Dec 15;143(12):3925-33.
Affiliations
- PMID: 2592763
Comparative Study
In vitro evidence that Langerhans cells can adopt two functionally distinct forms capable of antigen presentation to T lymphocytes
J W Streilein et al. J Immunol. 1989.
Abstract
Monodisperse suspensions of epidermal Langerhans cells (LC) have been examined for their capacity to process and present Ag immediately upon extraction from mouse epidermis (fresh LC) and after 72 h in tissue culture (cultured LC). Cultured, but not fresh, LC stimulated proliferation among autologous T cells, whereas fresh, but not cultured, LC proved to be superior at processing native OVA for presentation to an OVA peptide-specific, MHC-restricted T cell hybridoma. Cultured LC were also more effective at stimulating proliferation among allogeneic T cells. However, a significant, but difficult to quantify, component of lymphocyte activation in these assays was derived from the ability of cultured LC to stimulate autologous T cells. It has been proposed that the superior capacity of cultured LC to stimulate T cells in these assays is due to the "immaturity" of freshly prepared LC--which "mature" during the 72-h culture interval. Based on the observation that fresh LC are superior at processing native protein Ag, we would amend the currently held notion that there is a "precursor-product" relationship between fresh and cultured LC to include the fact that these populations are differentially equipped to carry out distinct physiologic functions and that fresh LC should not, therefore, be considered "immature." We propose that fresh LC (in vitro equivalents of intraepidermal LC) can process native protein Ag with great efficiency, and can present these Ag in situ to memory and effector T cells (high affinity TCR interactions). Cultured LC (in vitro equivalents of LC that have migrated from skin to draining lymph node) exchange highly efficient Ag processing for acquisition of accessory molecules (surface ligands and secreted cytokines) that promote activation of unprimed T cells (including even low affinity TCR interactions).
Similar articles
- Fresh and cultured Langerhans cells display differential capacities to activate hapten-specific T cells.
Dai R, Grammer SF, Streilein JW. Dai R, et al. J Immunol. 1993 Jan 1;150(1):59-66. J Immunol. 1993. PMID: 8417131 - Antigen processing and presentation by epidermal Langerhans cells. Induction of immunity or unresponsiveness.
Cruz PD Jr, Bergstresser PR. Cruz PD Jr, et al. Dermatol Clin. 1990 Oct;8(4):633-47. Dermatol Clin. 1990. PMID: 1979019 Review. - The ability of cultured Langerhans cells to process and present protein antigens is MHC-dependent.
Aiba S, Katz SI. Aiba S, et al. J Immunol. 1991 Apr 15;146(8):2479-87. J Immunol. 1991. PMID: 1849934 - Inhibition of Langerhans cell antigen-presenting function by IL-10. A role for IL-10 in induction of tolerance.
Enk AH, Angeloni VL, Udey MC, Katz SI. Enk AH, et al. J Immunol. 1993 Sep 1;151(5):2390-8. J Immunol. 1993. PMID: 8103065 - Functional dichotomy between Langerhans cells that present antigen to naive and to memory/effector T lymphocytes.
Streilein JW, Grammer SF, Yoshikawa T, Demidem A, Vermeer M. Streilein JW, et al. Immunol Rev. 1990 Oct;117:159-83. doi: 10.1111/j.1600-065x.1990.tb00572.x. Immunol Rev. 1990. PMID: 2258190 Review.
Cited by
- Comparison of antigen presentation by lymph node cells from protein and peptide-primed mice.
Hoyne GF, Callow MG, Kuo MC, Thomas WR. Hoyne GF, et al. Immunology. 1993 Jan;78(1):58-64. Immunology. 1993. PMID: 8436404 Free PMC article. - Ultrastructural studies bearing on the mechanism of UVB-impaired induction of contact hypersensitivity to DNCB in man.
Mommaas AM, Mulder AA, Vermeer M, Boom BW, Tseng C, Taylor JR, Streilein JW. Mommaas AM, et al. Clin Exp Immunol. 1993 Jun;92(3):487-93. doi: 10.1111/j.1365-2249.1993.tb03426.x. Clin Exp Immunol. 1993. PMID: 8513580 Free PMC article. - Constitutive and inducible expression of interleukin-6 by Langerhans cells and lymph node dendritic cells.
Cumberbatch M, Dearman RJ, Kimber I. Cumberbatch M, et al. Immunology. 1996 Apr;87(4):513-8. doi: 10.1046/j.1365-2567.1996.504577.x. Immunology. 1996. PMID: 8675203 Free PMC article. - Disappearance of certain acidic organelles (endosomes and Langerhans cell granules) accompanies loss of antigen processing capacity upon culture of epidermal Langerhans cells.
Stössel H, Koch F, Kämpgen E, Stöger P, Lenz A, Heufler C, Romani N, Schuler G. Stössel H, et al. J Exp Med. 1990 Nov 1;172(5):1471-82. doi: 10.1084/jem.172.5.1471. J Exp Med. 1990. PMID: 2230653 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials