Purification and characterization of two cellobiohydrolases from Chaetomium thermophile var. coprophile - PubMed (original) (raw)

Purification and characterization of two cellobiohydrolases from Chaetomium thermophile var. coprophile

R K Ganju et al. Biochim Biophys Acta. 1989.

Abstract

Cellobiohydrolases I and II were purified to homogeneity from culture filtrates of a thermophilic fungus, Chaetomium thermophile var. coprophile, by using a combination of ion-exchange and gel filtration chromatographic procedures. The molecular weights of cellobiohydrolase I and II were estimated to be 60,000 and 40,000 and the enzymes were found to be glycoproteins containing 17 and 22.8% carbohydrate, respectively. The two forms differed in their amino-acid composition mainly with respect to threonine, alanine, methionine and arginine. Antibodies produced against either form of cellobiohydrolases failed to cross-react with the other. The tryptic maps of the two enzymes were found to be different. The temperature optima for cellobiohydrolase I and II were 75 and 70 degrees C, and they were optimally active at pH 5.8 and 6.4, respectively. Both enzymes were stable at higher temperatures and were able to degrade crystalline cellulosic materials.

PubMed Disclaimer

Similar articles

• Purification and characterization of two exo-cellobiohydrolases from the brown-rot fungus Coniophora puteana (Schum ex Fr) Karst.
  Schmidhalter DR, Canevascini G. Schmidhalter DR, et al. Arch Biochem Biophys. 1993 Feb 1;300(2):551-8. doi: 10.1006/abbi.1993.1076. Arch Biochem Biophys. 1993. PMID: 8434936
• [Purification and characterization of a cellobiohydrolase from the thermophilic fungus Chaetomium thermophilus CT2].
  Li YL, Li DC, Teng FC. Li YL, et al. Wei Sheng Wu Xue Bao. 2006 Feb;46(1):143-6. Wei Sheng Wu Xue Bao. 2006. PMID: 16579483 Chinese.
• [Purification and properties of an endocellulase from the thermophilic fungus Chaetomium thermophile].
  Lu M, Li D, Zhang C. Lu M, et al. Wei Sheng Wu Xue Bao. 2002 Aug;42(4):471-7. Wei Sheng Wu Xue Bao. 2002. PMID: 12557555 Chinese.
• Enzymes of carbohydrate metabolism of mycelial fungi from marine environments. beta-1,3-glucanase of the marine fungus Chaetomium indicum.
  Burtseva YV, Sova VV, Pivkin MV, Zvyagintseva TN. Burtseva YV, et al. Biochemistry (Mosc). 2000 Oct;65(10):1175-83. Biochemistry (Mosc). 2000. PMID: 11092961
• The beta-glucosidase system of the thermophilic fungus Chaetomium thermophile var. coprophile n. var.
  Lusis AJ, Becker RR. Lusis AJ, et al. Biochim Biophys Acta. 1973 Nov 2;329(1):5-16. doi: 10.1016/0304-4165(73)90003-2. Biochim Biophys Acta. 1973. PMID: 4784485 No abstract available.

Cited by

• Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum.
  Morag E, Halevy I, Bayer EA, Lamed R. Morag E, et al. J Bacteriol. 1991 Jul;173(13):4155-62. doi: 10.1128/jb.173.13.4155-4162.1991. J Bacteriol. 1991. PMID: 2061292 Free PMC article.
• Thermophilic fungi: their physiology and enzymes.
  Maheshwari R, Bharadwaj G, Bhat MK. Maheshwari R, et al. Microbiol Mol Biol Rev. 2000 Sep;64(3):461-88. doi: 10.1128/MMBR.64.3.461-488.2000. Microbiol Mol Biol Rev. 2000. PMID: 10974122 Free PMC article. Review.
• Characterization and substrate specificity of an endo-beta-1,4-D-glucanase I (Avicelase I) from an extracellular multienzyme complex of Bacillus circulans.
  Kim CH. Kim CH. Appl Environ Microbiol. 1995 Mar;61(3):959-65. doi: 10.1128/aem.61.3.959-965.1995. Appl Environ Microbiol. 1995. PMID: 7793925 Free PMC article.
• Global Transcriptome Characterization and Assembly of the Thermophilic Ascomycete Chaetomium thermophilum.
  Singh A, Schermann G, Reislöhner S, Kellner N, Hurt E, Brunner M. Singh A, et al. Genes (Basel). 2021 Sep 29;12(10):1549. doi: 10.3390/genes12101549. Genes (Basel). 2021. PMID: 34680944 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database