DNA methylation temporal profiling following peripheral versus central nervous system axotomy - PubMed (original) (raw)

Figure 6. Signalmap snapshot views of methylated promoter regions.

Snapshot views from Signalmap of methylated promoter regions show the transcription start site (TSS), primary transcript, CpG islands (CGI) and methylation data of these regions from the samples (SNA, Sham, DCA, Sham and Naïve) (Ensemble database 02–2007). The 5 tracks show the scaled and centered log2-values for each oligonucleotide probe (representing IP- to-Input enrichment ratios, normalized to the bi-weight mean methylation of the whole array), red bars in each track indicate _P_-values of greater + 2 or smaller − 2, which is supposed to be significantly enriched for the specific probe (log2-values). (a,b) Classical imprinted promoters; Plag1 (12,777,600 to 12,784,100 bp), Gnas (173,923,000 to 173,986,000 bp) and Nnat (157,245,600 to 157,262,000 bp), as well as germline genes; Dazl (49,749,600 to 49,764,000 bp) and Brdt (107,564,400 to 107,577,900 bp) show validation that the array used here detects methylation in and surrounding CGI. Y-axis log2 scale from + 2 to − 2. (c) Dio3 (110,723,200 to 110,730,600 bp) and Snrpb (129,866,800 to 129,874,400 bp) are two representative methylated genes we observed in our array that are also found to be methylated in the literature in mice. Y-axis log2 scale from + 5 to − 3. (d) Klf4 (55,547,800 to 55,558,200 bp) and Dnmt3b (153,336,000 to 153,345,600 bp) are two examples of methylated CGIs that have been previously shown to be involved in neurite outgrowth. Y-axis log2 scale from + 5 to − 3.