Regulation of RNA polymerase III transcription in response to F9 embryonal carcinoma stem cell differentiation - PubMed (original) (raw)

Regulation of RNA polymerase III transcription in response to F9 embryonal carcinoma stem cell differentiation

R J White et al. Cell. 1989.

Abstract

B2 genes are rodent-specific middle repetitive elements transcribed by RNA polymerase III. They are expressed in the ectoderm and mesoderm but not in the embryonic or extraembryonic endoderm of early mouse embryos. This tissue specificity is mimicked in vitro by embryonal carcinoma and embryonic stem cell lines. Nuclear run-on experiments show that the down-regulation of B2 genes during F9 embryonal carcinoma cell differentiation into endoderm occurs at the transcriptional level and that other class III genes, including those encoding tRNA, show a similar response. We have used cell-free extracts to investigate the molecular mechanisms responsible. The specific down-regulation of transcription by RNA polymerase III during F9 cell differentiation is due to a reduction in the activity of the general class III transcription factor TFIIIB.

PubMed Disclaimer

Similar articles

• Sox7 plays crucial roles in parietal endoderm differentiation in F9 embryonal carcinoma cells through regulating Gata-4 and Gata-6 expression.
  Futaki S, Hayashi Y, Emoto T, Weber CN, Sekiguchi K. Futaki S, et al. Mol Cell Biol. 2004 Dec;24(23):10492-503. doi: 10.1128/MCB.24.23.10492-10503.2004. Mol Cell Biol. 2004. PMID: 15542856 Free PMC article.
• Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
  Murphy S, Pierani A, Scheidereit C, Melli M, Roeder RG. Murphy S, et al. Cell. 1989 Dec 22;59(6):1071-80. doi: 10.1016/0092-8674(89)90763-0. Cell. 1989. PMID: 2532066
• Induction of B2 RNA polymerase III transcription by heat shock: enrichment for heat shock induced sequences in rodent cells by hybridization subtraction.
  Fornace AJ Jr, Mitchell JB. Fornace AJ Jr, et al. Nucleic Acids Res. 1986 Jul 25;14(14):5793-811. doi: 10.1093/nar/14.14.5793. Nucleic Acids Res. 1986. PMID: 2426659 Free PMC article.
• The transcription of B2 repeated sequences is regulated during the transition from quiescent to proliferative state in cultured rodent cells.
  Lania L, Pannuti A, La Mantia G, Basilico C. Lania L, et al. FEBS Lett. 1987 Jul 27;219(2):400-4. doi: 10.1016/0014-5793(87)80260-0. FEBS Lett. 1987. PMID: 3609298
• Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.
  Dumay-Odelot H, Durrieu-Gaillard S, Da Silva D, Roeder RG, Teichmann M. Dumay-Odelot H, et al. Cell Cycle. 2010 Sep 15;9(18):3687-99. doi: 10.4161/cc.9.18.13203. Epub 2010 Sep 1. Cell Cycle. 2010. PMID: 20890107 Free PMC article. Review.

Cited by

• The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance.
  Kessler AC, Maraia RJ. Kessler AC, et al. Nucleic Acids Res. 2021 Dec 2;49(21):12017-12034. doi: 10.1093/nar/gkab1145. Nucleic Acids Res. 2021. PMID: 34850129 Free PMC article.
• Effects on prostate cancer cells of targeting RNA polymerase III.
  Petrie JL, Swan C, Ingram RM, Frame FM, Collins AT, Dumay-Odelot H, Teichmann M, Maitland NJ, White RJ. Petrie JL, et al. Nucleic Acids Res. 2019 May 7;47(8):3937-3956. doi: 10.1093/nar/gkz128. Nucleic Acids Res. 2019. PMID: 30820548 Free PMC article.
• Biosynthesis of brain cytoplasmic 200 RNA.
  Kim Y, Lee J, Shin H, Jang S, Kim SC, Lee Y. Kim Y, et al. Sci Rep. 2017 Jul 31;7(1):6884. doi: 10.1038/s41598-017-05097-3. Sci Rep. 2017. PMID: 28761139 Free PMC article.
• Human MAF1 targets and represses active RNA polymerase III genes by preventing recruitment rather than inducing long-term transcriptional arrest.
  Orioli A, Praz V, Lhôte P, Hernandez N. Orioli A, et al. Genome Res. 2016 May;26(5):624-35. doi: 10.1101/gr.201400.115. Epub 2016 Mar 3. Genome Res. 2016. PMID: 26941251 Free PMC article.
• Regulation of pol III transcription by nutrient and stress signaling pathways.
  Moir RD, Willis IM. Moir RD, et al. Biochim Biophys Acta. 2013 Mar-Apr;1829(3-4):361-75. doi: 10.1016/j.bbagrm.2012.11.001. Epub 2012 Nov 16. Biochim Biophys Acta. 2013. PMID: 23165150 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Miscellaneous

  • NCI CPTAC Assay Portal