Dietary Protein to Carbohydrate Ratio and Caloric Restriction: Comparing Metabolic Outcomes in Mice - PubMed (original) (raw)

Comparative Study

Dietary Protein to Carbohydrate Ratio and Caloric Restriction: Comparing Metabolic Outcomes in Mice

Samantha M Solon-Biet et al. Cell Rep. 2015.

Abstract

Both caloric restriction (CR) and low-protein, high-carbohydrate (LPHC) ad-libitum-fed diets increase lifespan and improve metabolic parameters such as insulin, glucose, and blood lipids. Severe CR, however, is unsustainable for most people; therefore, it is important to determine whether manipulating macronutrient ratios in ad-libitum-fed conditions can generate similar health outcomes. We present the results of a short-term (8 week) dietary manipulation on metabolic outcomes in mice. We compared three diets varying in protein to carbohydrate ratio under both CR and ad libitum conditions. Ad libitum LPHC diets delivered similar benefits to CR in terms of levels of insulin, glucose, lipids, and HOMA, despite increased energy intake. CR on LPHC diets did not provide additional benefits relative to ad libitum LPHC. We show that LPHC diets under ad-libitum-fed conditions generate the metabolic benefits of CR without a 40% reduction in total caloric intake.

Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

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Figures

Figure. 1

Figure. 1. Food and energy intake ± SEM

(a) Average food intake (g/day), (b) energy intake (kJ/day) and (c) body weight (g) over 8 weeks of feeding in AL and CR regimes. Note that CR animals were offered exactly 40% of AL counterparts fed the same diet composition (HPLC, MPMC or LPHC). See also Table S2.

Figure 2

Figure 2. Metabolic phenotype ± SEM

The effect of diets on (a) insulin, (b) HOMA, (c) triglycerides and (d) HDLc and (e) oral glucose tolerance tests. (AL, ad libitum; CR, caloric restricted; HPLC, high ratio of protein to carbohydrate; MPMC, medium protein to carbohydrate ratio; LPHC, low protein to carbohydrate ratio). See also Table S2 and Table S3.

Figure 3

Figure 3. Hepatic and pancreatic pathology

Representative figures are shown of livers stained with hemotoxylin and eosin (a-b), scanning electron microscopy of the liver sinusoidal endothelium (c-d) and glucagon stains of the pancreatic islets (e-f). (g) The effect of diets pancreatic glucagon staining. Percentages indicate the proportion of samples with high intensity staining. (AL ad libitum; CR caloric restricted; HPLC high ratio of protein to carbohydrate; MPMC medium protein to carbohydrate ratio; LPHC low protein to carbohydrate ratio; P-values provided in Table S2). See also Figure S1 and Table S2.

Figure 4

Figure 4. Indirect calorimetry ± SEM

The effect of diets on (a) RER, (b) energy expenditure and (c) body fat. (AL ad libitum; CR caloric restricted; HPLC high ratio of protein to carbohydrate; MPMC medium protein to carbohydrate ratio; LPHC low protein to carbohydrate ratio. See also Table S2.

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References

    1. Ajala O, English P, Pinkney J. Systematic review and meta-analysis of different dietary approaches to the management of type 2 diabetes. Am J Clin Nutr. 2013;97:505–516. - PubMed
    1. Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H, Ross SE, Mostoslavsky R, Cohen HY, et al. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 2004;303:2011–2015. - PubMed
    1. Cogger VC, O'Reilly J, Warren A, Le Couteur DG. A standardized method for the analysis of liver sinusoidal endothelial cells and their fenestrations by scanning electron microscopy. J Vis Exp. 2015:e52698. doi: 10.3791/52698. - DOI - PMC - PubMed
    1. Dietrich P, Hellerbrand C. Non-alcoholic fatty liver disease, obesity and the metabolic syndrome. Best Pract Res Clin Gastroenterol. 2014;28:637–653. - PubMed
    1. Eisenstein AB, Strack I, Steiner A. Glucagon stimulation of hepatic gluconeogenesis in rats fed a high-protein, carbohydrate-free diet. Metab Clin Exp. 1974;23:15–23. - PubMed

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