Spatiotemporal expression and transcriptional perturbations by long noncoding RNAs in the mouse brain - PubMed (original) (raw)

Fig. 1.

LncRNAs are temporally regulated in the developing and adult brain. β-Gal staining of lncRNA heterozygous mutant brain coronal sections at E14.5 (A, C, E, and G) and adult (B, D, F, and H) stages. (A) Lincenc1 is expressed in the VTel and in the IZ and lateral CP (Inset) of the DTel at E14.5. (B, Upper Right) In the adult brain, Lincenc1 is expressed in the primary somatosensory cortex, more specifically in layers II/III of the S1 S1Tr and layer IV in the S1BF. (Lower Right) β-Gal staining also shows expression in the hippocampus (Left) and the dentate gyrus. (C and D) At E14.5, Eldr is expressed in the VTel (C), but in the adult brain it is expressed in scattered cells in the upper layers of the neocortex as well as in layer VI cells lining the subcortical white matter (arrowheads) (D, Left, Right, and Inset). (E) No β-gal staining could be detected in Tug1 +/− brains at E14.5, except in the choroid plexus. (F, Left, Right, and Inset) In adult brains, Tug1 expression was detected in scattered cells in the neocortex. (G and H) β-Gal staining indicates expression of Peril in the VTel at E14.5 (G) and in the ependymal zone of the ventricles in the adult brain (H, Right and Inset). Images are representative of staining performed on two animals. CP, cortical plate; DG, dentate gyrus; DTel, dorsal telencephalon; Hip, hippocampus; IZ, intermediate zone; LV, lateral ventricle; Ncx, neocortex; S1BF, primary somatosensory cortex barrel field; S1Tr, primary somatosensory of the trunk; Str, striatum; VTel, ventral telencephalon; VZ/SVZ, ventricular zone/subventricular zone. [Scale bars: 500 μm (A_–_H, lower magnification panels), 100 μm (B, D, F, H, higher magnification right panels and inset).]