Fungus-mediated synthesis of silver nanoparticles and evaluation of antitumor activity - PubMed (original) (raw)

Fungus-mediated synthesis of silver nanoparticles and evaluation of antitumor activity

S M El-Sonbaty. Cancer Nanotechnol. 2013.

Abstract

Silver nanoparticles (AgNPs) were biologically synthesized using aqueous extract of Agaricus bisporus fungi. Physicochemical analysis of silver nanoparticles revealed that they are of spherical shape ranged size of 8-20 nm, and their zeta potential equal -7.23 mV. Silver nanoparticles showed a dose-dependent cytotoxic effect on MCF-7 breast cancer cells with LD50 (50 μg/ml). Mice bearing Ehrlich solid tumor treated with AgNPs and exposed to gamma radiation significantly ameliorated superoxide dismutase and catalase activity and reduced glutathione with an increase in malondialdehyde and nitric oxide levels compared to tumor group. Gamma radiation with AgNPs induced apoptotic cell count in Ehrlich solid tumor cells from 68.3 (treated with AgNPs) to 98.1 % (treated with AgNPs with gamma radiation) via a mechanism involved caspase-3. Histological sections of tumor tissue of mice treated with AgNPs showed antiangiogenesis effect of AgNPs. The overall result indicates that AgNPs synergize with gamma radiation, promising a potential combined therapy of cancer.

Keywords: Apoptosis; Caspase; Cell cycle; Ehrlich tumor; Silver nanoparticles.

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Figures

Fig. 1

Fig. 1

TEM image of AgNPs showing a nanoparticles of spherical shape with diameter range between 8 and 20 nm

Fig. 2

Fig. 2

Percentage count of apoptotic cells of Ehrlich ascites tumor cells in response to AgNPs and gamma irradiation

Fig. 3

Fig. 3

Percentage count of Caspase-3 activated cells of Ehrlich ascites tumor cells in response to AgNPs and gamma irradiation

Fig. 4

Fig. 4

Photomicrograph of Ehrlich ascites tumor sections from mice. a Control section showing sheets of malignant cells with lot of dilated congested blood vessels (H&E ×400). b and c Section in the Ehrlich ascites tumor that received AgNPs showing malignant cells with necrotic and apoptotic cells and reduced blood vessels (H&E ×200 and 400, respectively). d Section from mice that received AgNPs and gamma radiation showing most cells in necrosis and apoptosis stage also fragmented cell constituents (H&E ×400)

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