Association of THBS1 rs1478605 T>C in 5'-untranslated regions with the development and progression of gastric cancer - PubMed (original) (raw)

Association of THBS1 rs1478605 T>C in 5'-untranslated regions with the development and progression of gastric cancer

Bin-Bin Hong et al. Biomed Rep. 2015 Mar.

Abstract

Thrombospondin 1 (THBS1) plays an important role in angiogenesis and tumor progression. The aim of the present study was to investigate the effects of single-nucleotide polymorphisms (rs1478605 and rs3743125) in the untranslated regions of the THBS1 gene on the development and progression of gastric cancer. In the case-control study, 275 gastric cancer patients and 275 cancer-free controls were successfully genotyped using polymerase chain reaction-restriction fragment length polymorphism. The data demonstrated that THBS1 rs1478605 genotypic distributions significantly differed between the patient and control groups (P=0.005). Carriers of the CC genotype exhibited a decreased risk of developing gastric cancer compared to the carriers of the CT and TT genotypes [adjusted odd ratio (OR), 0.56; 95% confidence interval (CI), 0.39-0.79; P=0.001]. The CC genotype of rs1478605 was negatively associated with gastric cancer lymph node metastasis (OR, 0.41; 95% CI, 0.23-0.71; P=0.001) and was associated with a reduced risk of lymph node metastasis in male patients (OR, 0.27; 95% CI, 0.14-0.52; P<0.001). The THBS1 CT haplotype was associated with a reduced risk of developing gastric cancer (OR, 0.56; 95% CI, 0.33-0.93; P=0.02). By contrast, no association was observed between THBS1 rs3743125 and the development and progression of gastric cancer. These results suggest that THBS1 rs1478605 represents a potential molecular marker for gastric cancer.

Keywords: THBS1; gastric cancer; single-nucleotide polymorphism.

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Figures

Figure 1.

Figure 1.

Genotyping patterns of rs1478605 and rs3743125. (A) rs1478605. Lane 1, DNA marker; lane 2, TT genotype [152 base pairs (bp) only]; lane 3, CT genotype (152+132+20 bp); lane 4, CC genotype (132+20 bp). (B) rs3743125: lane 1, DNA marker; lane 2, TT genotype (168 bp only); lane 3, CT genotype (168+145+23 bp); lane 4, CC genotype (145+23 bp).

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