The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing - PubMed (original) (raw)
doi: 10.1177/2211068215589580. Epub 2015 Jun 15.
David M Rissin 2, Cheuk W Kan 2, David R Fournier 2, Tomasz Piech 2, Todd G Campbell 2, Raymond E Meyer 2, Matthew W Fishburn 2, Carlos Cabrera 2, Purvish P Patel 2, Erica Frew 2, Yao Chen 2, Lei Chang 2, Evan P Ferrell 2, Volker von Einem 3, William McGuigan 4, Marcus Reinhardt 3, Heiko Sayer 3, Claus Vielsack 3, David C Duffy 2
Affiliations
- PMID: 26077162
- DOI: 10.1177/2211068215589580
The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing
David H Wilson et al. J Lab Autom. 2016 Aug.
Abstract
Disease detection at the molecular level is driving the emerging revolution of early diagnosis and treatment. A challenge facing the field is that protein biomarkers for early diagnosis can be present in very low abundance. The lower limit of detection with conventional immunoassay technology is the upper femtomolar range (10(-13) M). Digital immunoassay technology has improved detection sensitivity three logs, to the attomolar range (10(-16) M). This capability has the potential to open new advances in diagnostics and therapeutics, but such technologies have been relegated to manual procedures that are not well suited for efficient routine use. We describe a new laboratory instrument that provides full automation of single-molecule array (Simoa) technology for digital immunoassays. The instrument is capable of single-molecule sensitivity and multiplexing with short turnaround times and a throughput of 66 samples/h. Singleplex and multiplexed digital immunoassays were developed for 16 proteins of interest in cardiovascular, cancer, infectious disease, neurology, and inflammation research. The average sensitivity improvement of the Simoa immunoassays versus conventional ELISA was >1200-fold, with coefficients of variation of <10%. The potential of digital immunoassays to advance human diagnostics was illustrated in two clinical areas: traumatic brain injury and early detection of infectious disease.
Keywords: HD-1; Simoa; digital immunoassay; multiplexing; single-molecule array.
© 2015 Society for Laboratory Automation and Screening.
Similar articles
- A fully-automated, six-plex single molecule immunoassay for measuring cytokines in blood.
Rivnak AJ, Rissin DM, Kan CW, Song L, Fishburn MW, Piech T, Campbell TG, DuPont DR, Gardel M, Sullivan S, Pink BA, Cabrera CG, Fournier DR, Duffy DC. Rivnak AJ, et al. J Immunol Methods. 2015 Sep;424:20-7. doi: 10.1016/j.jim.2015.04.017. Epub 2015 May 7. J Immunol Methods. 2015. PMID: 25960176 Free PMC article. - Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA.
Yu ZT, Guan H, Cheung MK, McHugh WM, Cornell TT, Shanley TP, Kurabayashi K, Fu J. Yu ZT, et al. Sci Rep. 2015 Jun 15;5:11339. doi: 10.1038/srep11339. Sci Rep. 2015. PMID: 26074253 Free PMC article. - Evaluation of fully automated assays for the detection of Rubella IgM and IgG antibodies by the Elecsys(®) immunoassay system.
van Helden J, Grangeot-Keros L, Vauloup-Fellous C, Vleminckx R, Masset F, Revello MG. van Helden J, et al. J Virol Methods. 2014 Apr;199:108-15. doi: 10.1016/j.jviromet.2014.01.009. Epub 2014 Jan 30. J Virol Methods. 2014. PMID: 24487099 - Multiplexing molecular diagnostics and immunoassays using emerging microarray technologies.
Ling MM, Ricks C, Lea P. Ling MM, et al. Expert Rev Mol Diagn. 2007 Jan;7(1):87-98. doi: 10.1586/14737159.7.1.87. Expert Rev Mol Diagn. 2007. PMID: 17187487 Review. - Digital detection of proteins.
Duffy DC. Duffy DC. Lab Chip. 2023 Mar 1;23(5):818-847. doi: 10.1039/d2lc00783e. Lab Chip. 2023. PMID: 36625134 Review.
Cited by
- Associations of plasma phosphorylated tau181 and neurofilament light chain with brain amyloid burden and cognition in objectively defined subtle cognitive decline patients.
Huang Y, Li Y, Xie F, Guo Q. Huang Y, et al. CNS Neurosci Ther. 2022 Dec;28(12):2195-2205. doi: 10.1111/cns.13962. Epub 2022 Sep 8. CNS Neurosci Ther. 2022. PMID: 36074638 Free PMC article. - The relevance of BDNF for neuroprotection and neuroplasticity in multiple sclerosis.
Maiworm M. Maiworm M. Front Neurol. 2024 Aug 1;15:1385042. doi: 10.3389/fneur.2024.1385042. eCollection 2024. Front Neurol. 2024. PMID: 39148705 Free PMC article. Review. - Ultra-sensitive and rapid detection of nucleic acids and microorganisms in body fluids using single-molecule tethering.
Cheng WC, Horn T, Zayats M, Rizk G, Major S, Zhu H, Russell J, Xu Z, Rothman RE, Celedon A. Cheng WC, et al. Nat Commun. 2020 Sep 22;11(1):4774. doi: 10.1038/s41467-020-18574-7. Nat Commun. 2020. PMID: 32963224 Free PMC article. - Hypoxemia increases blood-brain barrier permeability during extreme apnea in humans.
Bailey DM, Bain AR, Hoiland RL, Barak OF, Drvis I, Hirtz C, Lehmann S, Marchi N, Janigro D, MacLeod DB, Ainslie PN, Dujic Z. Bailey DM, et al. J Cereb Blood Flow Metab. 2022 Jun;42(6):1120-1135. doi: 10.1177/0271678X221075967. Epub 2022 Jan 21. J Cereb Blood Flow Metab. 2022. PMID: 35061562 Free PMC article. - Profiling individual clinical responses by high-frequency serum neurofilament assessment in MS.
Akgün K, Kretschmann N, Haase R, Proschmann U, Kitzler HH, Reichmann H, Ziemssen T. Akgün K, et al. Neurol Neuroimmunol Neuroinflamm. 2019 Apr 8;6(3):e555. doi: 10.1212/NXI.0000000000000555. eCollection 2019 May. Neurol Neuroimmunol Neuroinflamm. 2019. PMID: 31119188 Free PMC article.
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical