Multitarget super-resolution microscopy with high-density labeling by exchangeable probes - PubMed (original) (raw)

doi: 10.1038/nmeth.3466. Epub 2015 Jul 6.

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• PMID: 26147917
• DOI: 10.1038/nmeth.3466

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Multitarget super-resolution microscopy with high-density labeling by exchangeable probes

Tai Kiuchi et al. Nat Methods. 2015 Aug.

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Abstract

We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.

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