Asymmetric distribution of actin mRNA and cytoskeletal pattern generation in polarized epithelial cells - PubMed (original) (raw)

Asymmetric distribution of actin mRNA and cytoskeletal pattern generation in polarized epithelial cells

H Cheng et al. J Mol Biol. 1989.

Abstract

We analysed the distribution of actin mRNA in intestinal epithelial cells using in situ hybridization of 35S-labelled cytoplasmic beta-actin RNA. We found that the distribution of actin mRNA generally parallels that of polymerized actin, i.e. there is an accumulation of actin mRNA in the apical end of villous epithelial cells. Furthermore, the development of this asymmetric localization of actin mRNA appears to parallel the elaboration of the cytoskeleton during cellular differentiation. We discuss the possibility that the interaction between actin and its mRNA may be important for the establishment and maintenance of cytoskeletal pattern in polarized epithelial cells.

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