Reconsidering DNA Polymerases at the Replication Fork in Eukaryotes - PubMed (original) (raw)

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Reconsidering DNA Polymerases at the Replication Fork in Eukaryotes

Bruce Stillman. Mol Cell. 2015.

Abstract

The distribution of DNA polymerase activities at the eukaryotic DNA replication fork was "established," but recent genetic studies in this issue of Molecular Cell raise questions about which polymerases are copying the leading and lagging strand templates (Johnson et al, 2015).

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Figures

Figure 1. DNA Polymerases at the Eukaryotic DNA Replication Fork

(A) DNA polymerase δ synthesizes DNA during lagging (discontinuously synthesized, top) and leading (continuously synthesized, bottom) replication. (B) The prevailing model in which DNA polymerase δ synthesizes the lagging strand and polymerase ε the leading strand. (C) A potential new model in which DNA polymerase δ normally replicates both strands and, upon DNA damage in the leading strand template, a switch to polymerase ε occurs, linking DNA-damage detection to the essential role for polymerase ε and associated checkpoint proteins. In all cases, DNA polymerase α is coupled with primase to synthesize a RNA-DNA primer on the lagging strand that is recognized by RFC and PCNA to switch to the replicative polymerase. PCNA couples other events at the replication fork, such as nucleosome assembly.

Comment on

A Major Role of DNA Polymerase δ in Replication of Both the Leading and Lagging DNA Strands.
Johnson RE, Klassen R, Prakash L, Prakash S. Johnson RE, et al. Mol Cell. 2015 Jul 16;59(2):163-175. doi: 10.1016/j.molcel.2015.05.038. Epub 2015 Jul 2. Mol Cell. 2015. PMID: 26145172 Free PMC article.

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Reconsidering DNA Polymerases at the Replication Fork in Eukaryotes - PubMed (original) (raw)