Cytocam-IDF (incident dark field illumination) imaging for bedside monitoring of the microcirculation - PubMed (original) (raw)

Cytocam-IDF (incident dark field illumination) imaging for bedside monitoring of the microcirculation

Guclu Aykut et al. Intensive Care Med Exp. 2015 Dec.

Abstract

Background: Orthogonal polarized spectral (OPS) and sidestream dark field (SDF) imaging video microscope devices were introduced for observation of the microcirculation but, due to technical limitations, have remained as research tools. Recently, a novel handheld microscope based on incident dark field illumination (IDF) has been introduced for clinical use. The Cytocam-IDF imaging device consists of a pen-like probe incorporating IDF illumination with a set of high-resolution lenses projecting images on to a computer controlled image sensor synchronized with very short pulsed illumination light. This study was performed to validate Cytocam-IDF imaging by comparison to SDF imaging in volunteers.

Methods: This study is a prospective, observational study. The subjects consist of 25 volunteers.

Results: Sublingual microcirculation was evaluated using both techniques. The main result was that Cytocam-IDF imaging provided better quality images and was able to detect 30% more capillaries than SDF imaging (total vessels density Cytocam-IDF: 21.60 ± 4.30 mm/mm(2) vs SDF: 16.35 ± 2.78 mm/mm(2), p < 0.0001). Comparison of the images showed increased contrast, sharpness, and image quality of both venules and capillaries.

Conclusions: Cytocam-IDF imaging detected more capillaries and provided better image quality than SDF imaging. It is concluded that Cytocam-IDF imaging may provide a new improved imaging modality for clinical assessment of microcirculatory alterations.

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Figures

Figure 1

Figure 1

Smaller SDF image in larger Cytocam-IDF image. This figure shows the field of view of SDF and Cytocam-IDF imaging superimposed on each other showing the larger field of view offered by the larger image sensor used by the later technique.

Figure 2

Figure 2

Histogram points taken for analyses; square capillary; circle venule; left Cytocam-IDF imaging; right SDF-imaging.

Figure 3

Figure 3

Boxplots of TVD and PVD.

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