A novel 3D method of locomotor analysis in adult zebrafish: Implications for automated detection of CNS drug-evoked phenotypes - PubMed (original) (raw)
Comparative Study
A novel 3D method of locomotor analysis in adult zebrafish: Implications for automated detection of CNS drug-evoked phenotypes
Adam Michael Stewart et al. J Neurosci Methods. 2015.
Abstract
Background: Expanding the spectrum of organisms to model human brain phenotypes is critical for our improved understanding of the pathobiology of neuropsychiatric disorders. Given the clear limitations of existing mammalian models, there is an urgent need for low-cost, high-throughput in-vivo technologies for drug and gene discovery.
New method: Here, we introduce a new automated method for generating 3D (X,Y,Z) swim trajectories in adult zebrafish (Danio rerio), to improve their neurophenotyping.
Results: Based on the Track3D module of EthoVision XT video tracking software (Noldus Information Technology), this tool enhances the efficient, high-throughput 3D analyses of zebrafish behavioral responses. Applied to adult zebrafish behavior, this 3D method is highly sensitive to various classes of psychotropic drugs, including selected psychostimulant and hallucinogenic agents.
Comparison with existing methods: Our present method offers a marked advance in the existing 2D and 3D methods of zebrafish behavioral phenotyping, minimizing research time and recording high-resolution, automatically synchronized videos with calculated, high-precision object positioning.
Conclusions: Our novel approach brings practical simplicity and 'integrative' capacity to the often complex and error-prone quantification of zebrafish behavioral phenotypes. Illustrating the value of 3D swim path reconstructions for identifying experimentally-evoked phenotypic profiles, this method fosters innovative, ethologically relevant, and fully automated small molecule screens using adult zebrafish.
Keywords: Drug discovery; High-throughput screening; Neurophenotype; Video tracking; Zebrafish.
Copyright © 2015 Elsevier B.V. All rights reserved.
Similar articles
- Automated high-throughput neurophenotyping of zebrafish social behavior.
Green J, Collins C, Kyzar EJ, Pham M, Roth A, Gaikwad S, Cachat J, Stewart AM, Landsman S, Grieco F, Tegelenbosch R, Noldus LP, Kalueff AV. Green J, et al. J Neurosci Methods. 2012 Sep 30;210(2):266-71. doi: 10.1016/j.jneumeth.2012.07.017. Epub 2012 Jul 31. J Neurosci Methods. 2012. PMID: 22884772 - Three-dimensional neurophenotyping of adult zebrafish behavior.
Cachat J, Stewart A, Utterback E, Hart P, Gaikwad S, Wong K, Kyzar E, Wu N, Kalueff AV. Cachat J, et al. PLoS One. 2011 Mar 7;6(3):e17597. doi: 10.1371/journal.pone.0017597. PLoS One. 2011. PMID: 21408171 Free PMC article. - Unique and potent effects of acute ibogaine on zebrafish: the developing utility of novel aquatic models for hallucinogenic drug research.
Cachat J, Kyzar EJ, Collins C, Gaikwad S, Green J, Roth A, El-Ounsi M, Davis A, Pham M, Landsman S, Stewart AM, Kalueff AV. Cachat J, et al. Behav Brain Res. 2013 Jan 1;236(1):258-269. doi: 10.1016/j.bbr.2012.08.041. Epub 2012 Sep 4. Behav Brain Res. 2013. PMID: 22974549 - Perspectives on zebrafish models of hallucinogenic drugs and related psychotropic compounds.
Neelkantan N, Mikhaylova A, Stewart AM, Arnold R, Gjeloshi V, Kondaveeti D, Poudel MK, Kalueff AV. Neelkantan N, et al. ACS Chem Neurosci. 2013 Aug 21;4(8):1137-50. doi: 10.1021/cn400090q. Epub 2013 Aug 6. ACS Chem Neurosci. 2013. PMID: 23883191 Free PMC article. Review. - Skin too thin? The developing utility of zebrafish skin (neuro)pharmacology for CNS drug discovery research.
Nguyen M, Poudel MK, Stewart AM, Kalueff AV. Nguyen M, et al. Brain Res Bull. 2013 Sep;98:145-54. doi: 10.1016/j.brainresbull.2013.08.004. Epub 2013 Sep 2. Brain Res Bull. 2013. PMID: 24007957 Review.
Cited by
- Histone Methyltransferase G9a Plays an Essential Role on Nicotine Preference in Zebrafish.
Faillace MP, Ortiz J, Rocco L, Bernabeu R. Faillace MP, et al. Mol Neurobiol. 2024 Sep;61(9):6245-6263. doi: 10.1007/s12035-024-03961-8. Epub 2024 Jan 30. Mol Neurobiol. 2024. PMID: 38289455 - Artificial Neural Network (ANN)-Based Pattern Recognition Approach Illustrates a Biphasic Behavioral Effect of Ethanol in Zebrafish: A High-Throughput Method for Animal Locomotor Analysis.
Myrov VO, Polovian AI, Kolchanova S, Galumov GK, Schiöth HB, Bozhko DV. Myrov VO, et al. Biomedicines. 2023 Dec 4;11(12):3215. doi: 10.3390/biomedicines11123215. Biomedicines. 2023. PMID: 38137436 Free PMC article. - Current State of Modeling Human Psychiatric Disorders Using Zebrafish.
Costa FV, Kolesnikova TO, Galstyan DS, Ilyin NP, de Abreu MS, Petersen EV, Demin KA, Yenkoyan KB, Kalueff AV. Costa FV, et al. Int J Mol Sci. 2023 Feb 6;24(4):3187. doi: 10.3390/ijms24043187. Int J Mol Sci. 2023. PMID: 36834599 Free PMC article. Review. - GoFish: A low-cost, open-source platform for closed-loop behavioural experiments on fish.
Ajuwon V, Cruz BF, Carriço P; Champalimaud Research Scientific Hardware Platform; Kacelnik A, Monteiro T. Ajuwon V, et al. Behav Res Methods. 2024 Jan;56(1):318-329. doi: 10.3758/s13428-022-02049-2. Epub 2023 Jan 9. Behav Res Methods. 2024. PMID: 36622558 Free PMC article. - A novel open-source raspberry Pi-based behavioral testing in zebrafish.
Li Y, Wu F, Wu Q, Liu W, Li G, Yao B, Xiao R, Hu Y, Wang J. Li Y, et al. PLoS One. 2022 Dec 27;17(12):e0279550. doi: 10.1371/journal.pone.0279550. eCollection 2022. PLoS One. 2022. PMID: 36574388 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources