High magnitude, in vitro, biaxial, cyclic tensile strain induces actin depolymerization in tendon cells - PubMed (original) (raw)
eCollection 2015 Apr-Jun.
Affiliations
- PMID: 26261792
- PMCID: PMC4496012
High magnitude, in vitro, biaxial, cyclic tensile strain induces actin depolymerization in tendon cells
Michael Lavagnino et al. Muscles Ligaments Tendons J. 2015.
Abstract
Background: the cytoskeleton is a dynamic arrangement of actin filaments that maintain cell shape and are vital in mediating the mechanobiological response of the cell.
Methods: to determine the cytoskeletal response to varying in vitro, biaxial stretch amplitudes, rat-tail tendon cells were paired into control and cyclically strained groups of 4.75, 9.5, or 12% strain at 1 Hz for 2 hours and the actin cytoskeleton stained. The cells were analyzed for actin staining intensity as a measure of relative depolymerization and for cell shape. Collagenase gene expression was measured in cells undergoing 12% cyclic strain at 1 Hz for 24 hours.
Results: there was no significant difference in the degree of actin staining intensity between the control group and cells strained at either 4.75 or 9.5%. However, cells strained at 12% demonstrated a significant decrease in actin staining intensity (depolymerization) compared to control cells, increased collagenase expression by 81%, and a clear shift towards a more rounded cell shape.
Conclusion: the results of this study demonstrate that the previously reported induction of collagenase activity associated with the application of high magnitude, in vitro, tensile strains may actually be a result of cytoskeletal depolymerization, which causes loss of tensional homeostasis and alteration of cell shape.
Keywords: actin intensity; cell shape; collagenase; mechanobiology; tendinopathy; under-stimulation.
Figures
Figure 1
Histogram of percentage of cells within each cell conformation range for 15% strain as well as control cells. A cell conformation of 1 corresponds to a round cell while higher values correspond to more elongate cells.
Figure 2
Representative photomicrographs of an elongate cell with intense fluorescence of actin stress fibers in the control group (A) and a round cell with less intense staining of the actin stress fibers in the 15% strain group (B).
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