Qa-2 expression in the adult murine thymus. A unique marker for a mature thymic subset - PubMed (original) (raw)
. 1989 Jan 1;142(1):48-56.
Affiliations
- PMID: 2642507
Qa-2 expression in the adult murine thymus. A unique marker for a mature thymic subset
J Vernachio et al. J Immunol. 1989.
Abstract
The MHC Ag, Qa-2, is expressed on all peripheral T cells, a subset of bone marrow cells, and to a lesser extent on B cells. The Qa-2 Ag is also expressed on 5 to 6% of normal adult murine thymocytes. Through the use of flow cytometry, counterflow centrifugal elutriation and acridine orange staining, we have analyzed the cell surface phenotype, cell size, and cell cycle status of this thymic population. Our studies indicate that Qa-2+ thymocytes are large, non-mitotic, G1 cells which have the cell surface phenotype of CD5+, CD3+, J11dLO and lack receptors for peanut agglutinin. This population can be further subdivided into three categories; CD4+/CD8-, CD4-/CD8+, and CD4-/CD8-. These data indicate that Qa-2 surface expression can only be detected on thymocytes in the final stages of differentiation. The Qa-2 Ag can be used as a cell surface marker to identify a unique subset of mature thymocytes.
Similar articles
- The stem cell antigens Sca-1 and Sca-2 subdivide thymic and peripheral T lymphocytes into unique subsets.
Spangrude GJ, Aihara Y, Weissman IL, Klein J. Spangrude GJ, et al. J Immunol. 1988 Dec 1;141(11):3697-707. J Immunol. 1988. PMID: 2460547 - The majority of CD4+8- thymocytes are functionally immature.
Ramsdell F, Jenkins M, Dinh Q, Fowlkes BJ. Ramsdell F, et al. J Immunol. 1991 Sep 15;147(6):1779-85. J Immunol. 1991. PMID: 1679836 - T cell antigen receptor expression by subsets of Ly-2-L3T4- (CD8-CD4-) thymocytes.
Wilson A, Ewing T, Owens T, Scollay R, Shortman K. Wilson A, et al. J Immunol. 1988 Mar 1;140(5):1470-6. J Immunol. 1988. PMID: 2450128 - Bovine T cell markers: anomalies or paradigms?
Higgins DA. Higgins DA. Dev Comp Immunol. 1984 Spring;8(2):257-71. doi: 10.1016/0145-305x(84)90033-8. Dev Comp Immunol. 1984. PMID: 6376189 Review. No abstract available. - Lectin receptors as lymphocyte surface markers.
Sharon N. Sharon N. Adv Immunol. 1983;34:213-98. doi: 10.1016/s0065-2776(08)60380-6. Adv Immunol. 1983. PMID: 6356810 Review. No abstract available.
Cited by
- Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen.
Ulker N, Lewis KD, Hood LE, Stroynowski I. Ulker N, et al. EMBO J. 1990 Dec;9(12):3839-47. doi: 10.1002/j.1460-2075.1990.tb07602.x. EMBO J. 1990. PMID: 2249652 Free PMC article. - Recognition of N-glycosidic carbohydrates on esophageal carcinoma cells by macrophage cell line THP-1.
Takano R, Nose M, Kanno H, Nishihira T, Hiraizumi S, Kobata A, Kyogoku M. Takano R, et al. Am J Pathol. 1990 Aug;137(2):393-401. Am J Pathol. 1990. PMID: 2167012 Free PMC article. - Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells.
Park JH, Adoro S, Guinter T, Erman B, Alag AS, Catalfamo M, Kimura MY, Cui Y, Lucas PJ, Gress RE, Kubo M, Hennighausen L, Feigenbaum L, Singer A. Park JH, et al. Nat Immunol. 2010 Mar;11(3):257-64. doi: 10.1038/ni.1840. Epub 2010 Jan 31. Nat Immunol. 2010. PMID: 20118929 Free PMC article. - Requirement for p56lck tyrosine kinase activation in T cell receptor-mediated thymic selection.
Hashimoto K, Sohn SJ, Levin SD, Tada T, Perlmutter RM, Nakayama T. Hashimoto K, et al. J Exp Med. 1996 Sep 1;184(3):931-43. doi: 10.1084/jem.184.3.931. J Exp Med. 1996. PMID: 9064353 Free PMC article. - Regulation of SATB1 during thymocyte development by TCR signaling.
Gottimukkala KP, Jangid R, Patta I, Sultana DA, Sharma A, Misra-Sen J, Galande S. Gottimukkala KP, et al. Mol Immunol. 2016 Sep;77:34-43. doi: 10.1016/j.molimm.2016.07.005. Epub 2016 Jul 25. Mol Immunol. 2016. PMID: 27454343 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials