The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export - PubMed (original) (raw)

The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export

P M Gannon et al. J Bacteriol. 1989 Feb.

Abstract

The product of the secB gene is required for export of a subset of secreted proteins to the outer membrane and periplasm of Escherichia coli. Precursor maltose-binding protein (MBP) accumulates in the cytoplasm of secB-carrying mutants, but export of alkaline phosphatase is only minimally affected by secB mutations. When export of MBP-alkaline phosphatase hybrid proteins was analyzed in wild-type and secB-carrying mutant strains, the first third of mature MBP was sufficient to render export of the hybrid proteins dependent on SecB. Substitution of a signal sequence from a SecB-independent protein had no effect on SecB-dependent export. These findings show that the first third of mature MBP is capable of conferring export incompetence on an otherwise competent protein.

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References

    1. Genes Dev. 1987 Apr;1(2):185-96 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5216-20 - PubMed
    1. J Bacteriol. 1988 Jan;170(1):126-31 - PubMed
    1. EMBO J. 1987 Nov;6(11):3449-54 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1576-80 - PubMed

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