Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins - PubMed (original) (raw)
Affiliations
- PMID: 2644484
Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins
O Skalli et al. Lab Invest. 1989 Feb.
Abstract
We examined by immunofluorescence the distribution of vimentin, desmin, alpha-smooth muscle actin and alpha-sarcomeric actin in normal human soft tissues and in pathologic tissues containing myofibroblasts, including normally healing granulation tissue, hypertrophic scar, and fibromatosis. The pattern of actin isoforms was also documented biochemically by two-dimensional gel electrophoresis. Fibroblastic and/or myofibroblastic cells in each setting always expressed vimentin and never alpha-sarcomeric actin. Moreover, these cells showed an heterogeneous cytoskeletal composition which defined four phenotypes: (a) cells expressing only vimentin; (b) cells expressing vimentin, alpha-smooth muscle actin and desmin; (c) cells expressing vimentin and alpha-smooth muscle actin; and (d) cells expressing vimentin and desmin. Given this, two groups of lesions are distinguished: the first contains only vimentin cells and consists of normally healing granulation tissue, eschars and normally healed scars; the second contains vimentin cells admixed with variable proportions of vimentin, alpha-smooth muscle actin and desmin, vimentin and alpha-smooth muscle actin, and vimentin and desmin cells and consists of hypertrophic scars and fibromatoses. Immunogold electron microscopy showed that alpha-smooth muscle actin was present in a proportion of cells with ultrastructural features of myofibroblasts. Our findings suggest that contrary to myofibroblasts of normally healing granulation tissue and normally healed scars, myofibroblasts of pathologic conditions characterized by chronic retraction express always immunochemical features indicative of smooth muscle differentiation.
Similar articles
- Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors.
Schürch W, Skalli O, Seemayer TA, Gabbiani G. Schürch W, et al. Am J Pathol. 1987 Jul;128(1):91-103. Am J Pathol. 1987. PMID: 2440309 Free PMC article. - Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
Darby I, Skalli O, Gabbiani G. Darby I, et al. Lab Invest. 1990 Jul;63(1):21-9. Lab Invest. 1990. PMID: 2197503 - Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis.
Vyalov SL, Gabbiani G, Kapanci Y. Vyalov SL, et al. Am J Pathol. 1993 Dec;143(6):1754-65. Am J Pathol. 1993. PMID: 7504890 Free PMC article. - Electron microscopy in the study of myofibroblastic lesions.
Eyden B. Eyden B. Semin Diagn Pathol. 2003 Feb;20(1):13-24. Semin Diagn Pathol. 2003. PMID: 12693672 Review. - The myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research.
Eyden B. Eyden B. Ultrastruct Pathol. 2001 Jan-Feb;25(1):39-50. doi: 10.1080/019131201300004672. Ultrastruct Pathol. 2001. PMID: 11297318 Review.
Cited by
- The role of TGF-β and myofibroblasts in the arteritis of Kawasaki disease.
Shimizu C, Oharaseki T, Takahashi K, Kottek A, Franco A, Burns JC. Shimizu C, et al. Hum Pathol. 2013 Feb;44(2):189-98. doi: 10.1016/j.humpath.2012.05.004. Epub 2012 Sep 3. Hum Pathol. 2013. PMID: 22955109 Free PMC article. - Modulation of actin isoform expression in alveolar myofibroblasts (contractile interstitial cells) during pulmonary hypertension.
Kapanci Y, Burgan S, Pietra GG, Conne B, Gabbiani G. Kapanci Y, et al. Am J Pathol. 1990 Apr;136(4):881-9. Am J Pathol. 1990. PMID: 2183626 Free PMC article. - Desmin expression in mesangial cells and fibroblasts in vitro.
Ennulat D, Steffens WL, Brown SA. Ennulat D, et al. In Vitro Cell Dev Biol Anim. 1998 Jun;34(6):450-4. doi: 10.1007/s11626-998-0077-y. In Vitro Cell Dev Biol Anim. 1998. PMID: 9661047 No abstract available. - The monoclonal antibody GB 42--a useful marker for the differentiation of myofibroblasts.
Kohnen G, Castellucci M, Hsi BL, Yeh CJ, Kaufmann P. Kohnen G, et al. Cell Tissue Res. 1995 Aug;281(2):231-42. doi: 10.1007/BF00583392. Cell Tissue Res. 1995. PMID: 7648618 - Morphology of interstitial cells in the healthy kidney.
Kaissling B, Hegyi I, Loffing J, Le Hir M. Kaissling B, et al. Anat Embryol (Berl). 1996 Apr;193(4):303-18. doi: 10.1007/BF00186688. Anat Embryol (Berl). 1996. PMID: 8694267 Review.