Elevated recombination rates in transcriptionally active DNA - PubMed (original) (raw)
Elevated recombination rates in transcriptionally active DNA
B J Thomas et al. Cell. 1989.
Free article
Abstract
We have examined the effect of RNA polymerase II-dependent transcription on recombination between directly repeated sequences of the GAL10 gene in S. cerevisiae. Direct repeat recombination leading either to plasmid loss or conversion was examined in isogenic strains containing null mutations in the positive activator, GAL4, or the repressor, GAL80. A 15-fold increase in the rate of plasmid loss is observed in cells constitutively expressing the construct compared with cells that are not. Conversion events that retain the integrated plasmid are not stimulated by expression of the repeats. Northern analysis of strains containing plasmid inserts with various promoter mutations suggests that the stimulation in recombination is mediated by events initiating within the integrated plasmid sequences.
Similar articles
- Stimulation of mitotic recombination upon transcription from the yeast GAL1 promoter but not from other RNA polymerase I, II and III promoters.
Bratty J, Ferbeyre G, Molinaro C, Cedergren R. Bratty J, et al. Curr Genet. 1996 Nov;30(5):381-8. doi: 10.1007/s002940050146. Curr Genet. 1996. PMID: 8929389 - A positive selection for plasmid loss in Saccharomyces cerevisiae using galactose-inducible growth inhibitory sequences.
Kawahata M, Amari S, Nishizawa Y, Akada R. Kawahata M, et al. Yeast. 1999 Jan 15;15(1):1-10. doi: 10.1002/(SICI)1097-0061(19990115)15:1<1::AID-YEA335>3.0.CO;2-9. Yeast. 1999. PMID: 10028180 - Nucleotide excision repair in the yeast Saccharomyces cerevisiae: its relationship to specialized mitotic recombination and RNA polymerase II basal transcription.
Friedberg EC, Bardwell AJ, Bardwell L, Feaver WJ, Kornberg RD, Svejstrup JQ, Tomkinson AE, Wang Z. Friedberg EC, et al. Philos Trans R Soc Lond B Biol Sci. 1995 Jan 30;347(1319):63-8. doi: 10.1098/rstb.1995.0010. Philos Trans R Soc Lond B Biol Sci. 1995. PMID: 7746856 Review. - Analysis of intrachromosomal homologous recombination in mammalian cell, using tandem repeat sequences.
Lambert S, Saintigny Y, Delacote F, Amiot F, Chaput B, Lecomte M, Huck S, Bertrand P, Lopez BS. Lambert S, et al. Mutat Res. 1999 Apr 9;433(3):159-68. doi: 10.1016/s0921-8777(99)00004-x. Mutat Res. 1999. PMID: 10343649 Review.
Cited by
- Tight control of trehalose content is required for efficient heat-induced cell elongation in Candida albicans.
Serneels J, Tournu H, Van Dijck P. Serneels J, et al. J Biol Chem. 2012 Oct 26;287(44):36873-82. doi: 10.1074/jbc.M112.402651. Epub 2012 Sep 5. J Biol Chem. 2012. PMID: 22952228 Free PMC article. - Using the Cre-lox system to randomize target gene expression states and generate diverse phenotypes.
Niesner B, Maheshri N. Niesner B, et al. Biotechnol Bioeng. 2013 Oct;110(10):2677-86. doi: 10.1002/bit.24952. Epub 2013 Jun 3. Biotechnol Bioeng. 2013. PMID: 23733452 Free PMC article. - Orientia tsutsugamushi OtDUB Is Expressed and Interacts with Adaptor Protein Complexes during Infection.
Adcox HE, Berk JM, Hochstrasser M, Carlyon JA. Adcox HE, et al. Infect Immun. 2022 Dec 15;90(12):e0046922. doi: 10.1128/iai.00469-22. Epub 2022 Nov 14. Infect Immun. 2022. PMID: 36374099 Free PMC article. - Overexpression of DNA polymerase zeta reduces the mitochondrial mutability caused by pathological mutations in DNA polymerase gamma in yeast.
Baruffini E, Serafini F, Ferrero I, Lodi T. Baruffini E, et al. PLoS One. 2012;7(3):e34322. doi: 10.1371/journal.pone.0034322. Epub 2012 Mar 28. PLoS One. 2012. PMID: 22470557 Free PMC article. - Dual Repressive Function by Cip1, a Budding Yeast Analog of p21, in Cell-Cycle START Regulation.
Li P, Liu X, Hao Z, Jia Y, Zhao X, Xie D, Dong J, Zeng F. Li P, et al. Front Microbiol. 2020 Jul 9;11:1623. doi: 10.3389/fmicb.2020.01623. eCollection 2020. Front Microbiol. 2020. PMID: 32733430 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases