Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase - PubMed (original) (raw)

Comparative Study

. 1989 Mar 10;243(4896):1363-6.

doi: 10.1126/science.2646717.

Affiliations

• PMID: 2646717
• DOI: 10.1126/science.2646717

Comparative Study

Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase

J R Sampson et al. Science. 1989.

Abstract

An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.

PubMed Disclaimer

Similar articles

• Recognition of tRNAPhe by phenylalanyl-tRNA synthetase of Thermus thermophilus.
  Moor NA, Ankilova VN, Lavrik OI. Moor NA, et al. Eur J Biochem. 1995 Dec 15;234(3):897-902. doi: 10.1111/j.1432-1033.1995.897_a.x. Eur J Biochem. 1995. PMID: 8575450
• Recognition nucleotides for human phenylalanyl-tRNA synthetase.
  Nazarenko IA, Peterson ET, Zakharova OD, Lavrik OI, Uhlenbeck OC. Nazarenko IA, et al. Nucleic Acids Res. 1992 Feb 11;20(3):475-8. doi: 10.1093/nar/20.3.475. Nucleic Acids Res. 1992. PMID: 1741281 Free PMC article.
• Kinetic analysis of an E.coli phenylalanine-tRNA synthetase mutant.
  Goodman R, Schwartz I. Goodman R, et al. Nucleic Acids Res. 1988 Aug 11;16(15):7477-86. doi: 10.1093/nar/16.15.7477. Nucleic Acids Res. 1988. PMID: 3045758 Free PMC article.
• Molecular dissection of a transfer RNA and the basis for its identity.
  Hou YM, Francklyn C, Schimmel P. Hou YM, et al. Trends Biochem Sci. 1989 Jun;14(6):233-7. doi: 10.1016/0968-0004(89)90033-9. Trends Biochem Sci. 1989. PMID: 2669241 Review.
• The transfer RNA identity problem: a search for rules.
  Saks ME, Sampson JR, Abelson JN. Saks ME, et al. Science. 1994 Jan 14;263(5144):191-7. doi: 10.1126/science.7506844. Science. 1994. PMID: 7506844 Review.

Cited by

• Rational design of oligonucleotides for enhanced in vitro transcription of small RNA.
  Matsuda T, Hori H, Yamagami R. Matsuda T, et al. RNA. 2024 May 16;30(6):710-727. doi: 10.1261/rna.079923.123. RNA. 2024. PMID: 38423625
• The tRNA identity landscape for aminoacylation and beyond.
  Giegé R, Eriani G. Giegé R, et al. Nucleic Acids Res. 2023 Feb 28;51(4):1528-1570. doi: 10.1093/nar/gkad007. Nucleic Acids Res. 2023. PMID: 36744444 Free PMC article. Review.
• Evolutionary Adjustment of tRNA Identity Rules in Bacillariophyta for Recognition by an Aminoacyl-tRNA Synthetase Adds a Facet to the Origin of Diatoms.
  Igloi GL. Igloi GL. J Mol Evol. 2022 Apr;90(2):215-226. doi: 10.1007/s00239-022-10053-5. Epub 2022 Mar 24. J Mol Evol. 2022. PMID: 35325255 Free PMC article.
• tRNA elbow modifications affect the tRNA pseudouridine synthase TruB and the methyltransferase TrmA.
  Schultz SK, Kothe U. Schultz SK, et al. RNA. 2020 Sep;26(9):1131-1142. doi: 10.1261/rna.075473.120. Epub 2020 May 8. RNA. 2020. PMID: 32385137 Free PMC article.
• Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway.
  Han L, Guy MP, Kon Y, Phizicky EM. Han L, et al. PLoS Genet. 2018 Mar 29;14(3):e1007288. doi: 10.1371/journal.pgen.1007288. eCollection 2018 Mar. PLoS Genet. 2018. PMID: 29596413 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Other Literature Sources

  • The Lens - Patent Citations