Human hepatitis delta virus RNA subfragments contain an autocleavage activity - PubMed (original) (raw)
Human hepatitis delta virus RNA subfragments contain an autocleavage activity
H N Wu et al. Proc Natl Acad Sci U S A. 1989 Mar.
Abstract
Hepatitis delta virus (HDV) contains a single-stranded circular RNA genome of 1.7 kilobases. In this report we demonstrate that subfragments of HDV RNA can undergo autocatalytic cleavage. This cleavage requires at least 500 microM of Mg2+ or Ca2+, is not affected by varying the pH from 5.0 to 9.1, and occurs with RNA fragments as small as 133 nucleotides. The larger RNA fragments containing additional HDV sequences have a lower efficiency of cleavage. Deletion analysis at both ends of RNA subfragments suggested that the catalytic ability of HDV RNA resides in a stretch of no more than 117 nucleotides around the cleavage site. The cleavage occurs at the phosphodiester bond between nucleotides 688 and 689 on the HDV genomic map, generating a 5' fragment with a terminal uridyl 2',3'-cyclic monophosphate residue and a 3' fragment with a guanosyl residue with a 5'-hydroxyl group. The smallest autocleaving RNA does not contain the "hammerhead" sequence required for the autocleavage of other known self-cleaving RNA. The cleavage of HDV RNA occurs at a much faster rate, even at a very low Mg2+ concentration, than that of other "ribozymes." Thus, HDV RNA represents a distinct class of ribozyme.
Similar articles
- Ribozyme cleavage of a 2,5-phosphodiester linkage: mechanism and a restricted divalent metal-ion requirement.
Shih IH, Been MD. Shih IH, et al. RNA. 1999 Sep;5(9):1140-8. doi: 10.1017/s1355838299990763. RNA. 1999. PMID: 10496215 Free PMC article. - Sequence and structure of the catalytic RNA of hepatitis delta virus genomic RNA.
Wu HN, Wang YJ, Hung CF, Lee HJ, Lai MM. Wu HN, et al. J Mol Biol. 1992 Jan 5;223(1):233-45. doi: 10.1016/0022-2836(92)90728-3. J Mol Biol. 1992. PMID: 1731072 - Structural and sequence elements required for the self-cleaving activity of the hepatitis delta virus ribozyme.
Thill G, Vasseur M, Tanner NK. Thill G, et al. Biochemistry. 1993 Apr 27;32(16):4254-62. doi: 10.1021/bi00067a013. Biochemistry. 1993. PMID: 8476853 - Self-cleaving ribozymes of hepatitis delta virus RNA.
Been MD, Wickham GS. Been MD, et al. Eur J Biochem. 1997 Aug 1;247(3):741-53. doi: 10.1111/j.1432-1033.1997.00741.x. Eur J Biochem. 1997. PMID: 9288893 Review. - HDV ribozymes.
Been MD. Been MD. Curr Top Microbiol Immunol. 2006;307:47-65. doi: 10.1007/3-540-29802-9_3. Curr Top Microbiol Immunol. 2006. PMID: 16903220 Review.
Cited by
- Structure and sequence at an RNA template 5' end influence insertion of transgenes by an R2 retrotransposon protein.
Palm SM, Horton CA, Zhang X, Collins K. Palm SM, et al. RNA. 2024 Aug 16;30(9):1227-1245. doi: 10.1261/rna.080031.124. RNA. 2024. PMID: 38960642 Free PMC article. - Inhibition of Cpeb3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs and enhances object location memory.
Chen CC, Han J, Chinn CA, Rounds JS, Li X, Nikan M, Myszka M, Tong L, Passalacqua LFM, Bredy T, Wood MA, Luptak A. Chen CC, et al. Elife. 2024 Feb 6;13:e90116. doi: 10.7554/eLife.90116. Elife. 2024. PMID: 38319152 Free PMC article. - Architectures and complex functions of tandem riboswitches.
Sherlock ME, Higgs G, Yu D, Widner DL, White NA, Sudarsan N, Sadeeshkumar H, Perkins KR, Mirihana Arachchilage G, Malkowski SN, King CG, Harris KA, Gaffield G, Atilho RM, Breaker RR. Sherlock ME, et al. RNA Biol. 2022 Jan;19(1):1059-1076. doi: 10.1080/15476286.2022.2119017. RNA Biol. 2022. PMID: 36093908 Free PMC article. - Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability.
Pacin-Ruiz B, Cortese MF, Tabernero D, Sopena S, Gregori J, García-García S, Casillas R, Najarro A, Aldama U, Palom A, Rando-Segura A, Galán A, Vila M, Riveiro-Barciela M, Quer J, González-Aseguinolaza G, Buti M, Rodríguez-Frías F. Pacin-Ruiz B, et al. Viruses. 2022 Jan 22;14(2):215. doi: 10.3390/v14020215. Viruses. 2022. PMID: 35215809 Free PMC article. - Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers.
Climent-Catala A, Ouldridge TE, Stan GV, Bae W. Climent-Catala A, et al. ACS Synth Biol. 2022 Feb 18;11(2):562-569. doi: 10.1021/acssynbio.1c00580. Epub 2022 Feb 8. ACS Synth Biol. 2022. PMID: 35133150 Free PMC article.
References
- J Virol. 1988 Jun;62(6):1855-61 - PubMed
- Cell. 1987 Feb 13;48(3):535-43 - PubMed
- Nature. 1987 Sep 24-30;329(6137):343-6 - PubMed
- Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 - PubMed
- Proc Natl Acad Sci U S A. 1986 Nov;83(22):8774-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous